A simple immunohistochemical method for perinatal mammalian ovaries revealed different kinetics of oocyte apoptosis caused by DNA damage and asynapsis.

IF 2.1 4区生物学 Q4 CELL BIOLOGY

Histochemistry and Cell Biology Pub Date : 2025-02-17 DOI:10.1007/s00418-025-02358-5

Hiroshi Kogo, Akiko Iizuka-Kogo, Hanako Yamamoto, Maiko Ikezawa, Yukiko Tajika, Toshiyuki Matsuzaki

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引用次数: 0

Abstract

Oocytes with meiotic defects are assumed to be eliminated by apoptosis in the perinatal period. However, oocyte apoptosis caused by meiotic defects has not been well analyzed, partly because of the great technical demands of tissue sectioning perinatal ovaries. In the present study, we applied a squash method for immunohistochemical analysis of perinatal mouse ovaries as a substitute for tissue sectioning. As a result, we could show different kinetics of apoptosis caused by DMC1- and SPO11-deficiencies, indicating that DNA damage-induced apoptosis precedes asynapsis-induced apoptosis in mouse oocytes. Double-mutant analysis revealed that only asynapsis-induced apoptosis was significantly dependent on HORMAD2. The present method is simple, easy, and able to analyze a sufficient number of oocytes to detect infrequent events in a single specimen, accelerating detailed immunohistochemical analyses of mammalian ovaries during the fetal and perinatal periods.

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一种简单的围产期哺乳动物卵巢免疫组织化学方法揭示了DNA损伤和失配引起的卵母细胞凋亡的不同动力学。

有减数分裂缺陷的卵母细胞被认为在围产期通过细胞凋亡被消除。然而，由于围产期卵巢组织切片技术要求高，有关减数分裂缺陷引起的卵母细胞凋亡的研究尚未得到很好的分析。在本研究中，我们采用挤压法对围产期小鼠卵巢进行免疫组织化学分析，以代替组织切片。因此，我们可以显示DMC1-和spo11缺陷引起的细胞凋亡的不同动力学，这表明DNA损伤诱导的细胞凋亡在小鼠卵母细胞中先于失联诱导的细胞凋亡。双突变分析显示，只有asynapysis诱导的细胞凋亡显著依赖于HORMAD2。本方法简单、容易，能够分析足够数量的卵母细胞，以检测单个标本中的罕见事件，加速对胎儿和围产期哺乳动物卵巢的详细免疫组织化学分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Histochemistry and Cell Biology 生物-细胞生物学

CiteScore

4.90

自引率

8.70%

发文量

112

审稿时长

1 months

期刊介绍： Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.