{"title":"<i>BvBZR1</i> improves parenchyma cell development and sucrose accumulation in sugar beet (<i>Beta vulgaris</i> L.) taproot.","authors":"Ningning Li, Wei Wang, Xiaotong Guo, Yaqing Sun, Guolong Li, Shaoying Zhang","doi":"10.3389/fpls.2025.1495161","DOIUrl":null,"url":null,"abstract":"<p><p>BRASSINAZOLE-RESISTANT (BZR) transcription factors, key elements of brassinolide (BR) signal transduction, play an important role in regulating plant growth and development. However, little is known about the molecular regulatory mechanism of BZR in sugar beet taproot growth. In this study, <i>BvBZR1</i> expression was significantly induced by exogenous BR treatment. Transgenic sugar beet overexpressing <i>BvBZR1</i> exhibited a higher taproot diameter compared with the wild type, mainly due to a significant enhancement in the spacing between cambial rings by increasing the size and layers of parenchyma cells. <i>BvBZR1</i> regulated the expression of <i>BvCESA6</i>, <i>BvXTH33</i>, <i>BvFAD3</i>, and <i>BvCEL1</i> and enhanced cell wall metabolism to promote sugar beet taproot growth in parenchyma cells and the development of each cambium ring. In addition, <i>BvBZR1</i> overexpression significantly increased the accumulation of sucrose and soluble sugars in the taproot, which was attributed to its ability to regulate the expression of <i>BvSPS</i> and <i>BvINV</i> and improve the activity of BvSPS, BvSS-S, BvSS-C, and BvINV enzymes in each cambium ring and parenchyma cell in the sugar beet taproot. These results suggest that <i>BvBZR1</i> can regulate the expression of genes related to cell wall and sucrose metabolism, improve corresponding enzyme activity, and promote the development of each cambium ring and parenchyma cell, thereby promoting the growth and development of sugar beet taproots.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1495161"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830749/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1495161","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
BRASSINAZOLE-RESISTANT (BZR) transcription factors, key elements of brassinolide (BR) signal transduction, play an important role in regulating plant growth and development. However, little is known about the molecular regulatory mechanism of BZR in sugar beet taproot growth. In this study, BvBZR1 expression was significantly induced by exogenous BR treatment. Transgenic sugar beet overexpressing BvBZR1 exhibited a higher taproot diameter compared with the wild type, mainly due to a significant enhancement in the spacing between cambial rings by increasing the size and layers of parenchyma cells. BvBZR1 regulated the expression of BvCESA6, BvXTH33, BvFAD3, and BvCEL1 and enhanced cell wall metabolism to promote sugar beet taproot growth in parenchyma cells and the development of each cambium ring. In addition, BvBZR1 overexpression significantly increased the accumulation of sucrose and soluble sugars in the taproot, which was attributed to its ability to regulate the expression of BvSPS and BvINV and improve the activity of BvSPS, BvSS-S, BvSS-C, and BvINV enzymes in each cambium ring and parenchyma cell in the sugar beet taproot. These results suggest that BvBZR1 can regulate the expression of genes related to cell wall and sucrose metabolism, improve corresponding enzyme activity, and promote the development of each cambium ring and parenchyma cell, thereby promoting the growth and development of sugar beet taproots.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
