Metabolomic combined with transcriptome analysis revealed the improvement of strawberry fruit quality after potassium sulfate treatment

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-02-16 DOI:10.1016/j.plaphy.2025.109658

Zihui Zhang, Yuhan Guan, Zhuo Zhang, Zhihong Zhang, He Li

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引用次数: 0

Abstract

Potash fertilizer is important for improving fruit quality, but its specific moderating roles must be further explored. To accomplish this objective, we utilized metabolomics and transcriptomics analyses to reveal the changes in metabolites and differential genes after potassium sulfate treatment, and we determined that the treatment substantially enhanced the intrinsic and external quality of ‘Yanli’ (Fragaria ×ananassa Duch.). The results showed that 345 metabolites were found in wide metabolomics, with 115 up-regulated and 230 down-regulated, in which the primary metabolites were more sugars, and the secondary metabolites were more flavonoids, accounting for 20.26% of the metabolites. Sugar metabolomics revealed a substantial increase in fructose content of 34.2 mg g⁻¹ after potassium sulfate treatment. 2335 differentially expressed genes were found in the transcriptome. The KEGG enrichment scatter plot revealed that the more enriched pathways were metabolic pathways, starch and sucrose metabolism pathways, and flavonoid biosynthesis pathways. Combined transcriptome and metabolomics analyses showed that three genes, FaGal, FaINV and FaFK were highly influential in the sugar metabolic pathway, five candidate genes were identified in the anthocyanin metabolic pathway. This study revealed the regulatory mechanism of potassium sulfate treatment for improving strawberry fruit quality. Our findings provide an important basis for in-depth research on the mechanism of differentially expressed genes as well as substantial theoretical and practical guidance for the scientific and rational application of potash fertilizers in strawberry production.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.