Photoprotection strategies of 'Cabernet Sauvignon' with different rootstocks under combined high temperature and strong light stress

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-02-17 DOI:10.1016/j.plaphy.2025.109651

Wenchao Shi , Dongliang Zhang , Chunmei Zhu , Zhiyu Liu , Zongyi Zhang , Baolong Zhao , Junli Sun , Bo Yang

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Abstract

The photosynthetic pigment content, leaf photosynthesis, and chlorophyll fluorescence of ‘Cabernet Sauvignon (CS)' with different rootstocks were measured at 9:00, 15:00, and 21:00 for three consecutive days under high temperature and strong light (HTSL). HTSL led to the inactivation of the CS oxygen evolution complex (OEC), thylakoid dissociation, damage to the receptor and donor sides of photosystem II (PSII), and obstruction of electron transfer between PSII and photosystem I (PSI), which reduced photochemical activity. After grafting, rootstocks decreased the L-band, K-band, and G-band; increased the photosynthetic reaction center's performance index (PI_abs), the number of OEC centers, and electron transfer between PSII and PSI; and maintained the stability of electron transfer and the energy distribution of the CS leaf optical system under HTSL. Under the combined stress, the PI_abs and PI_total were much higher for the CS/HTH combination than for other combinations (9.55 and 8.52, respectively). As a rootstock, HTH actively down-regulated the capture of light energy by CS, and enhanced the photosynthetic performance of CS PSII and the electron transfer from primary quinone electron acceptor (Q_A) to secondary quinone electron acceptor (Q_B), which promoted a balanced energy distribution, and strengthened the connectivity between PSII and PSI. The rapid oxidation fraction (δMRfast/MRo), reduction fraction (δMRslow/MRo) and redox rate (Vox and Vred) of plastocyanin PC⁺ and P₇₀₀⁺ were also enhanced in this combination. TOPSIS entropy weight analysis proved that HTH as a rootstock enhanced the ability of CS to resist combined HTSL stress.

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赤霞珠不同砧木在高温强光联合胁迫下的光保护策略

在高温强光（HTSL）条件下，连续3天分别于9:00、15:00、21:00测定不同砧木‘赤霞珠’的光合色素含量、叶片光合作用和叶绿素荧光。HTSL导致CS析氧复合物（OEC）失活，类囊体解离，光系统II （PSII）受体和供体侧损伤，PSII与光系统I （PSI）之间的电子传递受阻，从而降低光化学活性。嫁接后砧木的l波段、k波段和g波段降低；增加了光合反应中心的性能指数（PIabs）、OEC中心数和PSII与PSI之间的电子转移量；维持了HTSL下CS叶片光学系统电子传递和能量分布的稳定性。在联合胁迫下，CS/HTH组合的PIabs和PItotal均显著高于其他组合（分别为9.55和8.52）。作为砧木，HTH主动下调CS对光能的捕获，提高CS PSII的光合性能和电子从一次醌电子受体（QA）向二次醌电子受体（QB）的转移，促进了能量分配的平衡，加强了PSII与PSI之间的连性。PC+和P700+的快速氧化分数（δMRfast/MRo）、还原分数（δMRslow/MRo）和氧化还原速率（Vox和Vred）也得到了提高。TOPSIS熵权分析证明，HTH作为砧木增强了CS抵抗HTSL联合胁迫的能力。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.