Chemical composition and in vitro antifungal activity of Wild thyme (Thymus serpyllum L.) Lamiaceae and Reindeer lichen (Cladonia stellaris) Cladoniaceae from Yakutia (Russia)

Pharmacological Research - Natural Products Pub Date : 2025-02-17 DOI:10.1016/j.prenap.2025.100185

S. Chimi Fotso , S.B. Kenmogne , Marianna I. Soloveva , Sargylana S. Kuzmina , NTah à AYon Moise , F.A.A. Toze

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Abstract

The aim of this study is to investigate the chemical composition and the in vitro antifungal activity of the methanolic extracts of two traditional medicinal plant from the South of Arctic Circle: Thymus serpyllum (TS) and Cladonia stellaris (CS). The methanolic extracts of the powders from TS and CS obtained after maceration were used to determine their bioactive potential (phytochemical screening, total polyphenol (TPC), total flavonoid (TFC), condensed tannin (CTC) contents, and UHPLC chemical structure identification). It emerged from the phytochemical screening that, both methanolic extracts form (TS) and (CS) contained alkaloids, flavonoids, coumarins, phenols, terpenoids, steroids and tannins), whilst (TS) extract exhibited the highest TPC, TFC and CTC contents with the values of (174. 50 ± 9. 45 mg EGA/100 g DM), (88. 70 ± 2. 45 mg EQ/100 g DM) (2. 50 ± 0. 50 mg EC/100 g DM), respectively. The antifungal activity was assessed by determining the minimum inhibitory concentration (MIC) using the microdilution method against four fungal species. The results obtained showed that (TS) and (CS) methanolic extracts displayed moderate activities, with MICs varying from 64 to 1024 µg/mL and from 64 to 512 µg/mL respectively, with the best activity observed with (TS) extract against A. niger compared to the tested substances. The UHPLC analysis of the extracts allowed the identification of some potential bioactive compounds, including hitherto described antifungal agents.

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野生百里香的化学成分及体外抗真菌活性研究叶青科和驯鹿地衣（Cladonia stellaris）

研究了北极圈以南两种传统药用植物胸腺草（thyymus serpyllum， TS）和龙葵（Cladonia stellaris， CS）甲醇提取物的化学成分和体外抗真菌活性。利用浸渍后的黄芪粉和黄芪粉的甲醇提取物测定其生物活性潜力（植物化学筛选、总多酚（TPC）、总黄酮（TFC）、缩合单宁（CTC）含量和UHPLC化学结构鉴定）。植物化学筛选结果表明，甲醇提取物（TS）和甲醇提取物（CS）均含有生物碱、黄酮类、香豆素、酚类、萜类、甾体和单宁，而甲醇提取物（TS）的TPC、TFC和CTC含量最高，为（174）。50 ± 9。45 mg EGA/100 g DM)；70年 ± 2。45 mg EQ/100 g DM)50 ± 0。50 mg EC/100 g DM)。采用微量稀释法测定其对4种真菌的最低抑菌浓度（MIC），评价其抑菌活性。结果表明，（TS）和（CS）甲醇提取物对黑曲霉的抑制作用中等，mic值分别为64 ~ 1024 µg/mL和64 ~ 512 µg/mL，其中（TS）提取物对黑曲霉的抑制作用最好。提取物的UHPLC分析允许鉴定一些潜在的生物活性化合物，包括迄今为止描述的抗真菌剂。

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Pharmacological Research - Natural Products

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