Enhanced degradation of petroleum hydrocarbons by immobilizing Acinetobacter

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-02-07 DOI:10.1016/j.bej.2025.109666

Jienan Li , Hua Zhang , Kuixi Mei , Leni Sun , Li Wang , Changhai Liang

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引用次数: 0

Abstract

The highly efficient elimination of petroleum pollution is essential for addressing environmental issues and social sustainability. A hydrocarbon-degrading bacterium SHC is isolated from petroleum-contaminated soil in Chengdu, China, to augment bioremediation efforts against crude oil contamination. According to the analysis of 16S rDNA sequences, strain SHC was identified as Acinetobacter. The degradation rate of 2 g/L crude oil by free bacterium reached 30 % within 7 days. Through the analysis of hydrocarbon biodegradation, strain SHC was found to biodegrade more long-chain n-alkanes (C19–C29). Further, we delve into utilizing agar as a microbial immobilization matrix, supplemented with nutrient salts and oil-absorbing materials, to foster the growth and activity of the bacterium in challenging environments characterized by high salinity and acidity. In the final immobilization bacterium, the degradation rate of 3 % salt concentration reaches 22 %, the degradation rate of 5 g/L crude oil is 45 %, and the degradation rate at a pH of 10 is 74 %, and was 59 % more effective than the free bacterium on the removal of crude oil under the same conditions. These findings strongly indicate that encapsulating Acinetobacter SHC can protect them against extreme conditions, bolstering bioremediation endeavors.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.