Tailored Anti‐miR Decorated Covalent Organic Framework Enables Electrochemical Detection of Salivary miRNAs for Mild Traumatic Brain Injury

IF 13 2区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Small Pub Date : 2025-02-17 DOI:10.1002/smll.202412107

Pranay Saha, David Skrodzki, Teresa Aditya, Parikshit Moitra, Maha Alafeef, Ketan Dighe, Matthew Molinaro, Steven D. Hicks, Dipanjan Pan

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引用次数: 0

Abstract

MicroRNAs (miRNAs) play pivotal role as biomarkers for various diseases, with salivary miRNAs offering a non‐invasive diagnostic tool. For mild traumatic brain injury (mTBI), salivary miRNAs like miR‐let7a, miR‐21, and miR‐30e show promise for early detection of subtle injuries lacking reliable indicators. To advance the detection of mTBI‐related salivary miRNAs, this study integrates anti‐miRNA and miRNA hybridization‐based sensing with the development of a nanoscale covalent‐organic framework (COF) platform. COFs, with their highly customizable structures, large surface area, and biocompatibility, serve as a versatile foundation for biosensing applications. Here, post‐synthetic modification (PSM) of COFs is introduced for essential covalent conjugation of streptavidin for further immobilization of methylene blue‐labeled and biotinylated Anti‐miRNAs. Furthermore, the layer‐by‐layer assembly of conductive polymers enhanced the biosensor's electrical performance, enabling ultrasensitive and multiplexed detection of salivary miRNAs. Validated with samples from mixed martial arts participants and confirmed by polymerase chain reaction (PCR), this COF‐based platform demonstrates robust accuracy and reliability. By combining COF functionalization with advanced electrode design, it offers a powerful, non‐invasive solution for early mTBI detection and broader biomedical applications.

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来源期刊

Small 工程技术-材料科学：综合

CiteScore

17.70

自引率

3.80%

发文量

1830

审稿时长

2.1 months

期刊介绍： Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments. With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology. Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.