The Effects of Simulated Cobalt-Chromium-Molybdenum Wear Particles on a Macrophage-Lymphocyte Co-Culture for Evaluating Cellular Corrosion.

IF 3.4 2区医学 Q1 ORTHOPEDICS

Journal of Arthroplasty Pub Date : 2025-02-14 DOI:10.1016/j.arth.2025.02.020

Madison N Brown, Danielle M Bryant, Bailey Bond, Harrison Smith, Richard A Smith, William M Mihalko

{"title":"The Effects of Simulated Cobalt-Chromium-Molybdenum Wear Particles on a Macrophage-Lymphocyte Co-Culture for Evaluating Cellular Corrosion.","authors":"Madison N Brown, Danielle M Bryant, Bailey Bond, Harrison Smith, Richard A Smith, William M Mihalko","doi":"10.1016/j.arth.2025.02.020","DOIUrl":null,"url":null,"abstract":"Background: This study examined the impact of simulated wear particles on inflammatory cell-induced corrosion (ICIC).Methods: A 30-day macrophage-lymphocyte co-culture experiment was conducted using American Society of Testing and Materials F1537 cobalt-chromium-molybdenum (CoCrMo) disks, with activators and CoCrMo particles added at none, low (1:10), medium (1:100), and high (1:500) cell-particle ratios. Supernatants collected on days 10 and 30 were analyzed for tissue necrosis factor alpha (TNFα) and interleukin 6 (IL-6) levels via enzyme-linked immunosorbent assay. Disks were examined for ICIC damage using scanning electron microscopy (SEM), and the oxygen percentage on their surfaces was analyzed with energy-dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectrometry (XPS).Results: Most disks showed damage consistent with ICIC. Day 10 TNFα was higher in medium and high particle groups compared to groups without particles, while IL-6 was unexpectedly lower in those groups. On day 30, the activated medium particle group showed higher IL-6 than the non-activated group. The EDS showed no significant differences in %O (P = 0.77), but XPS results indicated significant differences (P < 0.0001) at high particle concentrations.Conclusion: Overall, the data suggested that increased TNFα reflected a heightened inflammatory response, particles might temporarily inhibit IL-6 release, and there is likely a synergistic effect between activators and particles on cellular responses.","PeriodicalId":51077,"journal":{"name":"Journal of Arthroplasty","volume":" ","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Arthroplasty","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.arth.2025.02.020","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: This study examined the impact of simulated wear particles on inflammatory cell-induced corrosion (ICIC).

Methods: A 30-day macrophage-lymphocyte co-culture experiment was conducted using American Society of Testing and Materials F1537 cobalt-chromium-molybdenum (CoCrMo) disks, with activators and CoCrMo particles added at none, low (1:10), medium (1:100), and high (1:500) cell-particle ratios. Supernatants collected on days 10 and 30 were analyzed for tissue necrosis factor alpha (TNFα) and interleukin 6 (IL-6) levels via enzyme-linked immunosorbent assay. Disks were examined for ICIC damage using scanning electron microscopy (SEM), and the oxygen percentage on their surfaces was analyzed with energy-dispersive X-ray spectrometry (EDS) and X-ray photoelectron spectrometry (XPS).

Results: Most disks showed damage consistent with ICIC. Day 10 TNFα was higher in medium and high particle groups compared to groups without particles, while IL-6 was unexpectedly lower in those groups. On day 30, the activated medium particle group showed higher IL-6 than the non-activated group. The EDS showed no significant differences in %O (P = 0.77), but XPS results indicated significant differences (P < 0.0001) at high particle concentrations.

Conclusion: Overall, the data suggested that increased TNFα reflected a heightened inflammatory response, particles might temporarily inhibit IL-6 release, and there is likely a synergistic effect between activators and particles on cellular responses.

查看原文本刊更多论文

背景：本研究探讨了模拟磨损颗粒对炎性细胞诱导腐蚀（ICIC）的影响：本研究探讨了模拟磨损颗粒对炎症细胞诱导腐蚀（ICIC）的影响：使用美国材料与试验协会的 F1537 钴铬钼（CoCrMo）盘进行了为期 30 天的巨噬细胞-淋巴细胞共培养实验，在无细胞-颗粒比、低细胞-颗粒比（1:10）、中细胞-颗粒比（1:100）和高细胞-颗粒比（1:500）的情况下添加活化剂和 CoCrMo 颗粒。第 10 天和第 30 天收集的上清液通过酶联免疫吸附试验分析了组织坏死因子α（TNFα）和白细胞介素 6（IL-6）的水平。用扫描电子显微镜（SEM）检查磁盘的 ICIC 损伤情况，并用能量色散 X 射线光谱法（EDS）和 X 射线光电子能谱法（XPS）分析磁盘表面的氧百分比：结果：大多数磁盘显示出与 ICIC 一致的损伤。第 10 天，与无颗粒组相比，中颗粒组和高颗粒组的 TNFα 较高，而这些组的 IL-6 则出乎意料地较低。第 30 天，活化的中颗粒组比未活化组显示出更高的 IL-6。EDS 显示氧化率无显著差异（P = 0.77），但 XPS 结果表明高浓度颗粒组存在显著差异（P < 0.0001）：总之，数据表明 TNFα 的增加反映了炎症反应的加剧，颗粒可能会暂时抑制 IL-6 的释放，激活剂和颗粒之间可能会对细胞反应产生协同效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Arthroplasty 医学-整形外科

CiteScore

7.00

自引率

20.00%

发文量

734

审稿时长

48 days

期刊介绍： The Journal of Arthroplasty brings together the clinical and scientific foundations for joint replacement. This peer-reviewed journal publishes original research and manuscripts of the highest quality from all areas relating to joint replacement or the treatment of its complications, including those dealing with clinical series and experience, prosthetic design, biomechanics, biomaterials, metallurgy, biologic response to arthroplasty materials in vivo and in vitro.