Gastrointestinal anaerobes and Enterococcus faecalis promote Candida glabrata gastrointestinal colonization and organ dissemination

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2025-02-14 DOI:10.1016/j.jiac.2025.102658

Masahiro Abe , Tsuyoshi Sekizuka , Yoshitsugu Miyazaki

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引用次数: 0

Abstract

Background

Candida glabrata is a common causative pathogen of endogenous candidiasis. It is assumed that the gastrointestinal flora affects C. glabrata gastrointestinal colonization and organ dissemination in the gastrointestinal tract (GIT). However, no reports have yet described the relationships between C. glabrata and bacteria in the GIT. This study aimed to clarify these relationships using a mouse endogenous candidiasis model with cortisone acetate immunosuppression.

Methods

Dysbiosis was induced in the GIT by several antibiotic combinations, and then C. glabrata gastrointestinal colonization and organ dissemination were evaluated. Next, metagenomic sequencing analysis of the gastrointestinal flora was performed to identify bacteria associated with C. glabrata organ dissemination. Finally, coinfection experiments were performed using bacteria isolated from the mouse GIT.

Results

C. glabrata organ dissemination was significantly promoted using specific antibiotics regardless of the amount of colonization in the GIT. Metagenomic sequencing analysis of the gastrointestinal flora showed that Enterococcus species and anaerobes were significantly associated with enhanced organ dissemination, whereas Enterobacterales, such as Escherichia species and Klebsiella species, were associated with the suppression of organ dissemination. In coinfection experiments, Enterococcus faecalis and Faecalibaculum rodentium inoculation, but not either of them, increased C. glabrata organ dissemination without affecting gastrointestinal colonization.

Conclusions

Coinfection with gastrointestinal bacteria promoted C. glabrata organ dissemination, which would indicate that gastrointestinal flora could affect C. glabrata dissemination. Therefore, the gastrointestinal flora could be a target for intervention or treatment in clinical settings. Insights from this study would lead to better control of endogenous candidiasis focusing on the gastrointestinal flora.

查看原文本刊更多论文

胃肠道厌氧菌和粪肠球菌促进光假丝酵母胃肠道定植和器官传播。

背景：光念珠菌是一种常见的内源性念珠菌病致病菌。据推测，胃肠道菌群影响了光棘草胃肠道定植和胃肠道器官播散。然而，目前还没有报道描述在GIT中C. glabrata与细菌的关系。本研究旨在通过使用醋酸可的松免疫抑制小鼠内源性念珠菌病模型来澄清这些关系。方法：采用多种抗生素组合诱导胃肠道菌群失调，观察光斑梭菌胃肠道定殖和脏器播散情况。接下来，对胃肠道菌群进行宏基因组测序分析，以鉴定与光棘球蚴器官传播相关的细菌。最后，使用从小鼠GIT中分离的细菌进行联合感染实验。结果：使用特异性抗生素可显著促进裸棘球蚴器官播散，无论其在胃肠道中的定殖量如何。胃肠道菌群的宏基因组测序分析显示，肠球菌和厌氧菌与器官传播增强显著相关，而肠杆菌如埃希氏菌和克雷伯氏菌则与器官传播抑制相关。在联合感染实验中，接种粪肠球菌和鼠粪球菌，但两者均不接种，增加了光滑梭菌的器官传播，但不影响胃肠道定植。结论：胃肠道菌群的共同感染促进了光棘球蚴脏器的传播，说明胃肠道菌群可能影响光棘球蚴的传播。因此，胃肠道菌群可能成为临床干预或治疗的目标。从这项研究的见解将导致更好地控制内源性念珠菌病集中在胃肠道菌群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.