Synthesis and Characterization of a Strontium-Quercetin Complex and Its In Vitro and In Vivo Potential for Application in Bone Regeneration.

Abstract

Progress has been made toward developing therapies to treat bone-related diseases and defects caused by trauma. However, some of these therapies, such as administering strontium ranelate to treat osteoporosis, have significant side effects. In this context, designing new and safer strontium-based materials constitutes an important current challenge. Here, we have used quercetin as a platform to synthesize a new complex based on strontium and evaluate its activity in vitro and in vivo. First, we carried out strontium complexation with quercetin. Then, we employed Fourier transform infrared spectroscopy, nuclear magnetic resonance, and thermal gravimetric analysis to determine the chemical composition of the resulting complex as [(C₁₅H₇O₇)Sr₂]·6(H₂O), which was also supported by theoretical calculations. This complex enhanced osteogenic differentiation of a preosteoblastic cell line in vitro, which increased alkaline phosphatase activity and extracellular matrix mineralization. By using a periapical lesion model in mice, we tested whether treatment with this complex could regenerate bone defects in vivo and found that the lesions decreased after 7 days. Together, our data showed that the strontium-quercetin complex synthesized herein is a potential candidate for developing new bone regeneration therapies.