From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation

IF 3.9 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Microbiology and Biotechnology Pub Date : 2025-02-18 DOI:10.1007/s00253-025-13428-y

Katharina Oehlenschläger, Jan-Niklas Hengsbach, Marianne Volkmar, Roland Ulber

{"title":"From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation","authors":"Katharina Oehlenschläger, Jan-Niklas Hengsbach, Marianne Volkmar, Roland Ulber","doi":"10.1007/s00253-025-13428-y","DOIUrl":null,"url":null,"abstract":"The biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial applications. Despite its decline due to the rise of petrochemical alternatives, renewed interest has emerged due to the global shift towards sustainable energy sources and rising oil prices. This review highlights the challenges in the cultivation process of C. acetobutylicum, such as strain degeneration, solvent toxicity, and substrate costs, and presents recent advancements aimed at overcoming these issues. Detailed documentation of the entire cultivation process including cell conservation, pre-culture, and main culture is seen as a fundamental step to facilitate further progress in research. Key strategies to improve production efficiency were identified as controlling pH to facilitate the metabolic shift from acidogenesis to solventogenesis, employing in situ product removal techniques, and advancing metabolic engineering for improved solvent tolerance of C. acetobutylicum. Furthermore, the use of renewable resources, particularly lignocellulosic biomass, positions ABE fermentation as a viable solution for sustainable solvent production. By focusing on innovative research avenues, including co-cultivation and bioelectrochemical systems, the potential for C. acetobutylicum to contribute significantly to a bio-based economy can be realized.• Historical significance and revival of ABE fermentation with Clostridium acetobutylicum• Current challenges and innovative solutions in cultivating C. acetobutylicum• New avenues for enhancing productivity and sustainability","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"109 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-025-13428-y.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Microbiology and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s00253-025-13428-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial applications. Despite its decline due to the rise of petrochemical alternatives, renewed interest has emerged due to the global shift towards sustainable energy sources and rising oil prices. This review highlights the challenges in the cultivation process of C. acetobutylicum, such as strain degeneration, solvent toxicity, and substrate costs, and presents recent advancements aimed at overcoming these issues. Detailed documentation of the entire cultivation process including cell conservation, pre-culture, and main culture is seen as a fundamental step to facilitate further progress in research. Key strategies to improve production efficiency were identified as controlling pH to facilitate the metabolic shift from acidogenesis to solventogenesis, employing in situ product removal techniques, and advancing metabolic engineering for improved solvent tolerance of C. acetobutylicum. Furthermore, the use of renewable resources, particularly lignocellulosic biomass, positions ABE fermentation as a viable solution for sustainable solvent production. By focusing on innovative research avenues, including co-cultivation and bioelectrochemical systems, the potential for C. acetobutylicum to contribute significantly to a bio-based economy can be realized.

• Historical significance and revival of ABE fermentation with Clostridium acetobutylicum

• Current challenges and innovative solutions in cultivating C. acetobutylicum

• New avenues for enhancing productivity and sustainability

查看原文本刊更多论文

从预培养到溶剂：乙酰丁酸梭菌培养的最新趋势

利用醋酸丁酸梭菌（Clostridium acetobutylicum）通过ABE（丙酮-丁醇-乙醇）发酵生产丁醇已经有100多年的历史了，最初是由第一次世界大战期间对合成橡胶的需求推动的，后来是为了工业应用。尽管由于石化替代品的兴起而导致其下降，但由于全球转向可持续能源和油价上涨，重新出现了兴趣。本文综述了C. acetobutylicum在培养过程中面临的挑战，如菌株退化、溶剂毒性和底物成本，并介绍了旨在克服这些问题的最新进展。详细记录整个培养过程，包括细胞保存、预培养和主培养，被视为促进研究进一步进展的基础步骤。提高生产效率的关键策略是控制pH以促进从酸生成到溶剂生成的代谢转变，采用原位产物去除技术，以及推进代谢工程以提高C. acetobutylicum的溶剂耐受性。此外，利用可再生资源，特别是木质纤维素生物质，使ABE发酵成为可持续溶剂生产的可行解决方案。通过关注创新研究途径，包括共培养和生物电化学系统，可以实现C. acetobutylicum对生物经济做出重大贡献的潜力。•乙酰丁酸梭菌ABE发酵的历史意义和复兴•培养乙酰丁酸梭菌的当前挑战和创新解决方案•提高生产力和可持续性的新途径

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Microbiology and Biotechnology 工程技术-生物工程与应用微生物

CiteScore

10.00

自引率

4.00%

发文量

535

审稿时长

2 months

期刊介绍： Applied Microbiology and Biotechnology focusses on prokaryotic or eukaryotic cells, relevant enzymes and proteins; applied genetics and molecular biotechnology; genomics and proteomics; applied microbial and cell physiology; environmental biotechnology; process and products and more. The journal welcomes full-length papers and mini-reviews of new and emerging products, processes and technologies.