Thiago Romanos Benatti, Filipe Manoel Ferreira, Rodolfo Manoel Lemes da Costa, Mario Luiz Teixeira de Moraes, Aurélio Mendes Aguiar, Donizete da Costa Dias, José Wilacildo de Matos, Aline Cristina Miranda Fernandes, Mateus Chagas Andrade, Leandro de Siqueira, Itaraju Junior Baracuhy Brum, André Vieira do Nascimento, Yuri Tani Utsunomiya, José Fernando Garcia, Evandro Vagner Tambarussi
{"title":"Accelerating eucalypt clone selection pipeline via cloned progeny trials and molecular data.","authors":"Thiago Romanos Benatti, Filipe Manoel Ferreira, Rodolfo Manoel Lemes da Costa, Mario Luiz Teixeira de Moraes, Aurélio Mendes Aguiar, Donizete da Costa Dias, José Wilacildo de Matos, Aline Cristina Miranda Fernandes, Mateus Chagas Andrade, Leandro de Siqueira, Itaraju Junior Baracuhy Brum, André Vieira do Nascimento, Yuri Tani Utsunomiya, José Fernando Garcia, Evandro Vagner Tambarussi","doi":"10.1186/s13007-025-01342-3","DOIUrl":null,"url":null,"abstract":"<p><p>The high productivity of Eucalyptus spp. forest plantations is mainly due to advances in silvicultural techniques and genetic improvement associated with the potential that many species of the genus have for vegetative propagation. However, long reproduction cycles for forest species pose significant challenges for genetic progress via traditional breeding programs. Furthermore, there is often poor correlation between individual (seedling) performance in initial (progeny trials) and final (clonal trials) stages of the breeding program. In this scenario, cloned progeny trials (CPT) offer an alternative to accelerate the eucalypt clone selection pipeline, combining progeny and clonal trials in a single experiment. CPT has the potential to speed up the evaluation process and increase its efficiency by developing new commercial genotypes that were tested as clones from the initial stage of the breeding program. Thus, this study aims to assess the potential of CPT to accelerate eucalypt clone selection programs by estimating the genetic parameters, analyzing responses to selection, and predicting the adequate number of ramets to be used in CPT of Eucalyptus urophylla x Eucalyptus grandis. The results show that when the number of ramets per progeny was decreased from five to one there was a reduction in the estimates of broad-sense heritability and accuracy. However, three ramets/progeny can be used without significant reductions in these estimates. CPT accelerates clonal selection by combining progeny and clonal trial methodologies, enabling an evaluation of performance as both progeny and clone. This capacity is very important for vegetatively propagated crop species such as Eucalyptus. Integrating CPT with SNP markers can offer an alternative to shorten the tree clone selection pipeline, better estimate and decompose the genetic variance components, and improve the correlation between initial and final performance for selected genotypes. This study confirms the potential of CPT to improve selection processes and accelerate genetic gains in the eucalypt clone selection pipeline.</p>","PeriodicalId":20100,"journal":{"name":"Plant Methods","volume":"21 1","pages":"19"},"PeriodicalIF":4.7000,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827336/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Methods","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s13007-025-01342-3","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
The high productivity of Eucalyptus spp. forest plantations is mainly due to advances in silvicultural techniques and genetic improvement associated with the potential that many species of the genus have for vegetative propagation. However, long reproduction cycles for forest species pose significant challenges for genetic progress via traditional breeding programs. Furthermore, there is often poor correlation between individual (seedling) performance in initial (progeny trials) and final (clonal trials) stages of the breeding program. In this scenario, cloned progeny trials (CPT) offer an alternative to accelerate the eucalypt clone selection pipeline, combining progeny and clonal trials in a single experiment. CPT has the potential to speed up the evaluation process and increase its efficiency by developing new commercial genotypes that were tested as clones from the initial stage of the breeding program. Thus, this study aims to assess the potential of CPT to accelerate eucalypt clone selection programs by estimating the genetic parameters, analyzing responses to selection, and predicting the adequate number of ramets to be used in CPT of Eucalyptus urophylla x Eucalyptus grandis. The results show that when the number of ramets per progeny was decreased from five to one there was a reduction in the estimates of broad-sense heritability and accuracy. However, three ramets/progeny can be used without significant reductions in these estimates. CPT accelerates clonal selection by combining progeny and clonal trial methodologies, enabling an evaluation of performance as both progeny and clone. This capacity is very important for vegetatively propagated crop species such as Eucalyptus. Integrating CPT with SNP markers can offer an alternative to shorten the tree clone selection pipeline, better estimate and decompose the genetic variance components, and improve the correlation between initial and final performance for selected genotypes. This study confirms the potential of CPT to improve selection processes and accelerate genetic gains in the eucalypt clone selection pipeline.
期刊介绍：
Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.
There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics.
Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.
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