Use of Physical Methods for the Development of a Protocol for the Purification of Virus-Like Particles Based on the Bacteriophage MS2 Capsid Protein

IF 0.6 4区材料科学 Q4 CRYSTALLOGRAPHY

Crystallography Reports Pub Date : 2025-02-16 DOI:10.1134/S106377452460217X

P. V. Konarev, L. N. Borschevskaya, T. L. Gordeeva, E. B. Pichkur, G. S. Peters, S. P. Sineokiy, V. R. Samygina

引用次数: 0

Abstract

Samples of self-assembled particles based on the capsid protein of bacteriophage MS2 were obtained using different purification protocols and characterized. The study demonstrates the feasibility of using a combination of electron microscopy and small-angle X-ray scattering (SAXS) to select the optimal purification protocol. Electron microscopy allows the evaluation of self-assembly, while SAXS helps to estimate the statistical distribution of particles of different shapes and sizes in a sample. The best results were obtained using the PEG precipitation in combination with gel filtration and ultrafiltration.

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利用物理方法开发基于噬菌体MS2衣壳蛋白的病毒样颗粒纯化方案

研究人员采用不同的纯化方案获得了基于噬菌体 MS2 包囊蛋白的自组装颗粒样品，并对其进行了表征。该研究证明了结合使用电子显微镜和小角 X 射线散射（SAXS）来选择最佳纯化方案的可行性。电子显微镜可对自组装进行评估，而小角 X 射线散射则有助于估算样品中不同形状和大小颗粒的统计分布。将 PEG 沉淀与凝胶过滤和超滤结合使用可获得最佳结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crystallography Reports 化学-晶体学

CiteScore

1.10

自引率

28.60%

发文量

审稿时长

4-8 weeks

期刊介绍： Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.