Monitoring the Formation of Microcrystal Suspensions of Proteins Used in Serial Microcrystallography by Small-Angle X-ray Scattering

IF 0.6 4区材料科学 Q4 CRYSTALLOGRAPHY

Crystallography Reports Pub Date : 2025-02-16 DOI:10.1134/S1063774524602247

P. V. Konarev, G. S. Peters, V. R. Samygina

引用次数: 0

Abstract

Serial crystallography, which has been extensively developed in the past years, makes it possible to study dynamic processes occurring in protein molecules. The method is implemented at fourth-generation synchrotron sources or free electron lasers and it requires suspensions of microcrystals. It was demonstrated that small-angle X-ray scattering can be used to monitor the formation of such suspensions in crystallization conditions as exemplified by lysozyme.

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用小角度x射线散射监测序列微晶学中蛋白质微晶悬浮液的形成

序列晶体学在过去几年得到了广泛的发展，使研究蛋白质分子中的动态过程成为可能。该方法是在第四代同步加速器源或自由电子激光器上实现的，它需要微晶体悬浮液。结果表明，在溶菌酶的结晶条件下，小角度x射线散射可以用来监测这种悬浮液的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crystallography Reports 化学-晶体学

CiteScore

1.10

自引率

28.60%

发文量

审稿时长

4-8 weeks

期刊介绍： Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.