Magnetically controlled all-in-one sensing platform for triple-mode detection of organophosphorus pesticides using DNA tetrahedrons-polydopamine reporters

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-02-12 DOI:10.1016/j.bios.2025.117258

Haiwen Xiao, Jianing Ma, Zihan Liu, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang

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引用次数: 0

Abstract

Accurate and on-site detection of organophosphorus pesticides (OPs) in complex matrixes is important for environmental monitoring and food safety. Herein, a colorimetric (CL), photothermal (PT) and fluorescence (FL) triple-mode sensor was constructed for OPs detection based on magnetically controlled all-in-one platform, which was featured of specific recognition unit, abundant loading of DNA tetrahedrons-Fe³⁺-polydopamine (TDN-Fe³⁺-PDA) with peroxidase-like activity, and verifiable triple-mode signal output. Based on the aptamer-target recognition and efficient separation of MBs, large amount of TDN-Fe³⁺-PDA reporters was released. Taking full advantages of the supernatant and precipitation, OPs was sensitively detected with the TDN-Fe³⁺-PDA catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)-H₂O₂ system (CL mode), photothermal effect of TDN-Fe³⁺-PDA and oxidized TMB (oxTMB) (PT mode), and fluorescent signal of TDN-templated copper nanoclusters on MBs (FL mode). Using profenofos (Pro) as the model target, the triple-mode sensing platform realized sensitive detection of Pro with the detection limits 0.23 ng/mL, 0.40 ng/mL and 0.14 ng/mL for CL, PT and FL mode, respectively. The proposed strategy provided a simple and accurate method for on-site detection of OPs, holding promising application in environmental and food contamination monitoring.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.