Agglomeration behavior of oleosin Q647G5 in ethanol solution: Formation mechanism and interfacial properties of micron-sized aggregates

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-02-08 DOI:10.1016/j.foodhyd.2025.111189

Lei Wang , Pengzhan Liu , Guoqin Liu

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Abstract

Oleosin was a potential natural surfactant, however, the exploration of its structural and property has been hindered by the poor water solubility. In this study, the representative oleosin, Q647G5, was first prepared and its dissolution and agglomeration behavior in high concentration ethanol were investigated. The results demonstrated that solvent extraction could be a promising method for preparing oleosin, as the protein could be dissolved in a 65% ethanol solution while maintaining its native conformation. The aggregation behavior was assessed through light scattering and fluorescence spectroscopy, while the interfacial properties and behavior of oleosin Q647G5 were studied through molecular dynamics simulations and interfacial tension measurements. It was discovered that the protein can aggregate in ethanol due to strong hydrophobic interactions facilitated by its transmembrane domain, with larger aggregate sizes correlating with increased interfacial tension. These insights make valuable contributions to advancing the potential industrial applications of solubilized oleosin.

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.