Complete genomic analysis of tomato chlorosis virus isolates from Sichuan and Chongqing, China

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-02-11 DOI:10.1016/j.virol.2025.110447

Xiaolong Yang, Jie Li, Mingrui Gao, Puxin Huang, Mingjun Li, Gentu Wu, Ling Qing

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引用次数: 0

Abstract

Tomato chlorosis virus (ToCV) has recently emerged as a significant threat to crop production in China. In this study, we collected tomato leaf samples displaying symptoms of interveinal chlorosis, curling, and yellowing from Sichuan Province and Chongqing City. Reverse transcription polymerase chain reaction (RT-PCR) analysis confirmed that 3 out of 10 samples from Sichuan and 9 out of 24 samples from Chongqing were infected with ToCV. Complete genomic sequences of ToCV were assembled from the cDNA of two infected leaves (SC744 and CQ630), which were deposited in GenBank under accession numbers CQ630: PQ153240 and PQ153241 and SC744: PQ261056 and PQ261057. Phylogenetic analysis indicated that ToCV isolates from the Sichuan Province and Chongqing City were closely related to the ToCV-BJ isolate. The sequence identity matrix shows that p22 has the highest variability at 94.3%–98.4%. p6 is the most conserved, with identities between 98% and 100%. In RNA2, p4 varies from 93.9% to 100%, while Hsp70 remains highly conserved at 97.9%–99%. This study provides the first report on the complete genomic sequences of ToCV from Sichuan Province and Chongqing City, enhancing our understanding of ToCV distribution and genetic diversity in China.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.