Ultrasonic-assisted extraction (UAE) of Javanese turmeric rhizomes using natural deep eutectic solvents (NADES): Screening, optimization, and in vitro cytotoxicity evaluation

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-02-12 DOI:10.1016/j.ultsonch.2025.107271

Donna Maretta Ariestanti , Abdul Mun’im , Pietradewi Hartrianti , Basmah Nadia , Erika Chriscensia , Shereen Angelina Rattu , Redhalfi Fadhila , Anastacia Harianto , Adelina Simamora , Delly Ramadon , Richard Johari James , Fadlina Chany Saputri , Mitsuyasu Kato , Meidi Utami Puteri

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引用次数: 0

Abstract

Javanese turmeric (Curcuma xanthorrhiza Roxb.) is known for its diverse pharmacological activities due to its rich phytoconstituents, including curcuminoids and xanthorrhizol. Typically, these compounds are extracted using organic solvents, which pose health and environmental risks. Therefore, safer and more environmentally friendly green extraction methods are being developed. This study investigated the effect of ultrasound-assisted extraction (UAE) combined with natural deep eutectic solvents (NADES) based on choline chloride and organic acids (lactic, malic, and citric acid) to find the best combination for extracting curcuminoids and xanthorrhizol from Javanese turmeric. Results showed that UAE using choline chloride and malic acid (1:1) (ChCl-MA) yielded the best results. The Box–Behnken Design optimized water addition, solvent-to-powder ratio, and extraction time, with optimal conditions being 25 % water addition, a 20 mL/g ratio, and a 15-minute extraction time. This method yielded 4.58 mg/g of curcuminoids and 12.93 mg/g of xanthorrhizol. Furthermore, the ChCl-MA NADES with UAE extraction showed more cytoselective activity towards the HeLa cancer cell line compared to the non-cancer HaCaT cell line. In contrast, traditional ethanol extraction was non-selective, as indicated by similar cell viability reductions in both HeLa and HaCaT cells at 6.25 ppm. Collectively, this study is the first to report the optimal NADES combination with UAE, based on salts and organic acids, for the extraction of Javanese turmeric rhizomes with selective cytotoxic effects against cancer cells. These findings may contribute to the development of novel, naturally derived anticancer agents using green extraction techniques.

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超声波辅助提取爪哇姜黄根茎：天然深共晶溶剂（NADES）的筛选、优化及体外细胞毒性评价

爪哇姜黄（Curcuma xanthorrhiza Roxb.）因其丰富的植物成分（包括姜黄素和黄菌素）而具有多种药理活性。通常，这些化合物是用有机溶剂提取的，这对健康和环境构成风险。因此，人们正在开发更安全、更环保的绿色提取方法。本研究考察了超声辅助提取（UAE）与天然深共晶溶剂（NADES）联合提取氯化胆碱和有机酸（乳酸、苹果酸和柠檬酸）的效果，以寻找提取爪哇姜黄中姜黄素和黄菌根醇的最佳组合。结果表明，氯化胆碱与苹果酸（ChCl-MA） 1:1配比的UAE效果最佳。Box-Behnken设计优化了水的添加量、溶剂与粉末的比和提取时间，最佳条件为水的添加量为25%，比为20 mL/g，提取时间为15分钟。该方法的姜黄素提取率为4.58 mg/g，黄菌素提取率为12.93 mg/g。此外，与非肿瘤HaCaT细胞株相比，阿联酋提取物的ChCl-MA NADES对HeLa癌细胞表现出更强的细胞选择活性。相比之下，传统的乙醇提取是非选择性的，正如在6.25 ppm时HeLa和HaCaT细胞的细胞活力降低所表明的那样。总的来说，本研究首次报道了以盐和有机酸为基础的NADES与UAE的最佳组合，用于提取具有选择性细胞毒作用的爪哇姜黄根茎。这些发现可能有助于利用绿色提取技术开发新型、天然衍生的抗癌药物。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.