Accelerative effect of Carbazole nanocubosomes on the healing of methicillin-resistant Staphylococcus aureus-infected full-thickness wounds

IF 2.4 3区医学 Q2 DERMATOLOGY

Journal of tissue viability Pub Date : 2025-02-08 DOI:10.1016/j.jtv.2025.100869

Saba Samari , Mohammad Reza Farahpour , Zohreh Ghazi Tabatabaei

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引用次数: 0

Abstract

Background

Bacterial infections remain a major challenge in the treatment of infected wounds due to their resistance. Novel agents are urgently needed for effective treatment. This study aimed to evaluate the effects of carbazole nanocubosomes on the healing of methicillin-resistant Staphylococcus aureus (MRSA)-infected full-thickness wounds.

Materials and methods

Carbazole (Carbz) and cubosome/carbazole (Cubo/Carbz) structures were synthesized, and their physicochemical properties, toxicity, and in vitro antibacterial activities were assessed. The wound healing activity was evaluated by analyzing pathological parameters, including edema, immune cell infiltration, new vessel formation, fibroblast infiltration, and epithelial thickness. Additionally, the expression levels of TNF-α, Bcl-2, P-53, mTOR, cyclin D1, TGF-β, VEGF, and Col1A were measured to assess the effects of cubosomes on the wound healing process. The results confirmed the successful synthesis of cubosomes, along with the safety, antibacterial activity, and sustained release of Carbz.

Results

The administration of Carbz, particularly the Cubo/Carbz formulations, significantly accelerated the wound healing process by reducing bacterial counts, edema, immune cell infiltration, and the expression of Bcl-2 and TNF-α. Simultaneously, fibroblast infiltration, epithelial thickness, and the expression of P-53, mTOR, Col1A, cyclin D1, TGF-β, and VEGF were increased.

Conclusion

In conclusion, Cubo/Carbz accelerates the wound healing process by promoting apoptosis, reducing inflammation, and enhancing the expression of proliferative genes.

Abstract Image

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背景由于细菌的耐药性，细菌感染仍是治疗受感染伤口的一大挑战。目前迫切需要新型药物来进行有效治疗。本研究旨在评估咔唑纳米立方体对耐甲氧西林金黄色葡萄球菌（MRSA）感染的全厚伤口愈合的影响。材料与方法合成了咔唑（Carbz）和立方体/咔唑（Cubo/Carbz）结构，并评估了它们的理化性质、毒性和体外抗菌活性。通过分析水肿、免疫细胞浸润、新血管形成、成纤维细胞浸润和上皮厚度等病理参数来评估伤口愈合活性。此外，还测定了 TNF-α、Bcl-2、P-53、mTOR、细胞周期蛋白 D1、TGF-β、VEGF 和 Col1A 的表达水平，以评估立方体对伤口愈合过程的影响。结果通过减少细菌数量、水肿、免疫细胞浸润以及 Bcl-2 和 TNF-α 的表达，服用 Carbz（尤其是 Cubo/Carbz 配方）显著加速了伤口愈合过程。同时，成纤维细胞浸润、上皮厚度以及 P-53、mTOR、Col1A、细胞周期蛋白 D1、TGF-β 和血管内皮生长因子的表达均有所增加。

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来源期刊

Journal of tissue viability DERMATOLOGY-NURSING

CiteScore

3.80

自引率

16.00%

发文量

110

审稿时长

>12 weeks

期刊介绍： The Journal of Tissue Viability is the official publication of the Tissue Viability Society and is a quarterly journal concerned with all aspects of the occurrence and treatment of wounds, ulcers and pressure sores including patient care, pain, nutrition, wound healing, research, prevention, mobility, social problems and management. The Journal particularly encourages papers covering skin and skin wounds but will consider articles that discuss injury in any tissue. Articles that stress the multi-professional nature of tissue viability are especially welcome. We seek to encourage new authors as well as well-established contributors to the field - one aim of the journal is to enable all participants in tissue viability to share information with colleagues.