Competitive growth of Bacillus subtilis biofilms

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yumeng Fu , Shuaishuai Hu , Jiankun Wang , Xiaoling Wang
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引用次数: 0

Abstract

Competition for existence exists everywhere in nature. Competition includes competition between different species and competition between the same species. In this paper, Bacillus subtilis which is cultured on 1.5 wt% agar gel containing minimal salts glutamate glycerol (MSgg) medium is taken as the research object to explore the competitive behavior of the same Bacillus subtilis in the process of biofilm formation. Wide-field fluorescence microscopy is used to observe the competition of biofilms, which helps us obtain the evolvement of the edge features of the biofilm in the competition region and the distribution of different phenotypes. We find that the premature formation of the chain structure of the matrix producing cells in the competitive region restrict the outward expansion of the motile cells and thus result in the unique competition between the biofilms of Bacillus subtilis. Based on the microscopic experimental analysis of cell population collective motion during the biofilm growth, a cellular automata model of biofilm growth and evolvement is constructed. We simulate different behaviors of biofilm competition by adjusting the inoculation distance and parameters characterizing environmental pressure. The results show that the competition phenomenon of biofilm is due to the fact that matrix producing cells appear in the competition area in advance and form a barrier structure to prevent the movement and expansion of motile cells to the competition area.
枯草芽孢杆菌生物膜的竞争生长
生存竞争在自然界无处不在。竞争包括不同物种之间的竞争和同一物种之间的竞争。本文以枯草芽孢杆菌(Bacillus subtilis)为研究对象,在1.5 wt%含微量盐谷氨酸甘油(MSgg)的琼脂凝胶培养基上培养,探讨同种枯草芽孢杆菌在生物膜形成过程中的竞争行为。利用宽视场荧光显微镜观察生物膜的竞争,可以获得生物膜在竞争区域边缘特征的演变和不同表型的分布。研究发现,竞争区基质生成细胞链结构的过早形成限制了活动细胞的向外扩张,从而导致枯草芽孢杆菌生物膜之间的独特竞争。通过对生物膜生长过程中细胞群体集体运动的微观实验分析,建立了生物膜生长演化的细胞自动机模型。通过调整接种距离和环境压力表征参数,模拟了生物膜竞争的不同行为。结果表明,生物膜的竞争现象是由于产生基质的细胞提前出现在竞争区域,并形成屏障结构,阻止运动细胞向竞争区域移动和扩张。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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