In-depth Occlusion of Dentinal Tubules Induced by Polyaspartic Acid–Calcium and Magnesium Complexes

IF 5.7 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
D. Shen, H. Zhu, Y. Xu, Z. Zhou, Z. Wu, B. Fu
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引用次数: 0

Abstract

Dentin hypersensitivity has been widely recognized to be caused by patent dentinal tubules (DTs). A polyaspartic acid–calcium and magnesium (PAsp-Ca&Mg) complex process has been demonstrated to induce biomimetic mineralization of collagen fibrils. This study investigated the in vitro and in vivo occlusion of the DTs by a PAsp-Ca&Mg complex process. Dentin disks were treated by citric acid to open the DTs and randomly divided into 4 groups. Two experimental groups ( n = 18) were applied with PAsp-Ca&Mg suspension and phosphate solution or phosphate-fluoride solution in sequence for 10 min. Two positive control groups ( n = 18) were treated with Gluma or Duraphat. All the dentin disks were incubated in artificial saliva for 24 h. DT occlusion was characterized by scanning electron microscopy, and dentin permeability measurement was conducted regardless of being subject to abrasive or ultrasonic and acidic challenge. The incisors of 3 New Zealand rabbits were used to verify the in vivo occlusion of the DTs. In vitro and in vivo results demonstrated in-depth occlusion of the DTs to ~100 μm by the PAsp-Ca&Mg complex process. When compared with control groups, the PAsp-Ca&Mg complex process could significantly reduce dentin permeability ( P < 0.05) irrespective of abrasive or ultrasonic and acidic challenge. The PAsp-Ca&Mg complex process not only deeply occluded patent DTs but also significantly decreased dentin permeability and provided a paradigm for ion-doped DT occlusion. This provides a promising strategy for managing dentin hypersensitivity.
聚天冬氨酸-钙镁复合物致牙本质小管深度闭塞
牙本质过敏已被广泛认为是由牙本质小管未闭引起的。聚天冬氨酸-钙镁(PAsp-Ca&Mg)复合过程已被证明可诱导胶原原纤维的仿生矿化。本研究探讨了PAsp-Ca&;Mg复合物对DTs的体外和体内阻断作用。采用柠檬酸处理牙本质盘打开DTs,随机分为4组。两个实验组(n = 18)分别用PAsp-Ca&;Mg悬浮液和磷酸盐溶液或氟化磷酸盐溶液依次作用10 min。两个阳性对照组(n = 18)分别用Gluma或Duraphat处理。所有牙本质盘在人工唾液中孵育24 h,扫描电镜观察DT咬合的特征,并在磨料、超声和酸性刺激下测量牙本质渗透率。我们用3只新西兰兔的门牙来验证DTs在体内的咬合。体外和体内实验结果表明,PAsp-Ca&;Mg复合过程对DTs的深度阻断达到~100 μm。与对照组相比,PAsp-Ca&;Mg复合物过程可显著降低牙本质渗透性(P <;0.05),无论研磨或超声波和酸性挑战。PAsp-Ca&;Mg复合物过程不仅深度闭塞未愈合的DT,而且显著降低牙本质的通透性,为离子掺杂DT闭塞提供了范例。这为处理牙本质过敏提供了一个有前途的策略。
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来源期刊
Journal of Dental Research
Journal of Dental Research 医学-牙科与口腔外科
CiteScore
15.30
自引率
3.90%
发文量
155
审稿时长
3-8 weeks
期刊介绍: The Journal of Dental Research (JDR) is a peer-reviewed scientific journal committed to sharing new knowledge and information on all sciences related to dentistry and the oral cavity, covering health and disease. With monthly publications, JDR ensures timely communication of the latest research to the oral and dental community.
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