γδ17T Cells Aggravate Carcinogen-Induced Oral Squamous Cell Carcinoma

IF 5.7 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Y. Saba, S. Yacoub, Y. Netanely, Y. Jaber, R. Naamneh, K. Zubeidat, A. Meyer, Y.E. Shlomovitz, L. Eli-Berchoer, A. Wilensky, I. Prinz, A. Hovav
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Abstract

Oral squamous cell carcinoma (OSCC) is a highly aggressive malignancy, with a low 5-y survival rate and frequent local recurrence or metastasis. This study explores the role of γδT cells in the development and progression of OSCC. γδT cells, which exhibit innate and adaptive immune characteristics, are known for their dual role in cancer, acting as anti- and protumor agents depending on the context. Using a murine model of OSCC induced by the carcinogen 4-nitroquinoline-1-oxide (4NQO), which adequately mimics the progression of human OSCC, we investigated the impact of γδT cells on tumor growth and the tumor microenvironment. We first characterized the γδT cells of the tongue epithelium, the primary site for cancer development in this model. The results indicate that γδT cells are predominantly of the Vγ6+ subset, expanding postnatally in a microbiota-dependent manner. Upon 4NQO administration, depletion of γδT cells did not significantly alter the kinetics of OSCC progression but did result in a reduction in tumor size and number, suggesting a role in promoting tumor growth. Interestingly, the absence of IL-17, a key cytokine produced by the Vγ6+ subset, also resulted in reduced tumor volume without affecting disease progression, corroborating the protumor role of these cells in OSCC. Further analysis revealed that IL-17–producing γδT cells facilitate angiogenesis within the tumor microenvironment by promoting the expression of angiogenic factors. Of note, while 4NQO treatment increased the oral microbial load and altered its composition, IL-17 deficiency did not affect the oral microbiota, indicating that the effects of IL-17–producing γδT cells on OSCC are independent of microbial changes. This study highlights the pathologic role of IL-17–producing γδT cells in OSCC, particularly in promoting tumor growth through angiogenesis. This underscores the importance of γδT cells in OSCC and the need for further research into therapeutic strategies targeting these cells.
γδ17T细胞加重致癌物诱导的口腔鳞状细胞癌
口腔鳞状细胞癌(OSCC)是一种高度侵袭性的恶性肿瘤,5年生存率低,局部复发或转移频繁。本研究探讨γδT细胞在OSCC发生发展中的作用。γδT细胞表现出先天和适应性免疫特性,在癌症中具有双重作用,根据不同的环境分别作为抗肿瘤剂和抗肿瘤剂。采用模拟人类OSCC发生过程的致癌物质4-硝基喹啉-1-氧化物(4NQO)诱导的小鼠OSCC模型,研究了γδT细胞对肿瘤生长和肿瘤微环境的影响。我们首先表征了舌上皮的γδT细胞,这是该模型中癌症发展的原发部位。结果表明,γδT细胞主要为Vγ6+亚群,在出生后以微生物依赖的方式扩增。在给药4NQO后,γδT细胞的耗竭并没有显著改变OSCC进展的动力学,但确实导致肿瘤大小和数量的减少,表明其促进肿瘤生长的作用。有趣的是,IL-17(一种由v γ - 6+亚群产生的关键细胞因子)的缺失也导致肿瘤体积减小而不影响疾病进展,证实了这些细胞在OSCC中的肿瘤作用。进一步分析发现,产生il -17的γδT细胞通过促进血管生成因子的表达,促进肿瘤微环境内的血管生成。值得注意的是,虽然4NQO处理增加了口腔微生物负荷并改变了其组成,但IL-17缺乏并未影响口腔微生物群,这表明产生IL-17的γδT细胞对OSCC的影响与微生物变化无关。本研究强调了产生il -17的γδT细胞在OSCC中的病理作用,特别是通过血管生成促进肿瘤生长。这强调了γδT细胞在OSCC中的重要性,以及进一步研究针对这些细胞的治疗策略的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Dental Research
Journal of Dental Research 医学-牙科与口腔外科
CiteScore
15.30
自引率
3.90%
发文量
155
审稿时长
3-8 weeks
期刊介绍: The Journal of Dental Research (JDR) is a peer-reviewed scientific journal committed to sharing new knowledge and information on all sciences related to dentistry and the oral cavity, covering health and disease. With monthly publications, JDR ensures timely communication of the latest research to the oral and dental community.
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