Ankita Samal, Oliver D Kripfgans, I-Ching Wang, Amanda B Rodriguez Betancourt, Liana Webber, Carole Quesada, John Mazzocco, James D Wishart, Hsun-Liang Chan
{"title":"High-Frequency Ultrasound Characterization of Periodontal Soft Tissues Pre- and Post-Bacterial Inoculation.","authors":"Ankita Samal, Oliver D Kripfgans, I-Ching Wang, Amanda B Rodriguez Betancourt, Liana Webber, Carole Quesada, John Mazzocco, James D Wishart, Hsun-Liang Chan","doi":"10.1016/j.ultrasmedbio.2025.01.014","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Current diagnostic methods of inflammatory periodontal diseases, e.g., visual evaluation, periodontal probing, and radiographs, are either subjective or insensitive. Intra-oral high-frequency ultrasound was investigated to quantify inflammation by detecting tissue dimensional and perfusion changes.</p><p><strong>Methods: </strong>A cohort of 15-month-old mini-pigs, 4 female/male each, was analyzed. Pre-molars (PM) 3 and 4, as well as first molars (M1), were scanned. In bi-weekly time intervals all 4 quadrants were randomly enrolled and bacterial injection followed each quadrant scan in a weekly fashion. Soft tissue dimensions were obtained from B-mode images and statistically analyzed to identify correlations to inoculation time, i.e., response to bacterial loading, tooth type and sex, using analysis of variance and regression analysis. Color flow velocity and power-weighted color pixel density was obtained and statistically analyzed analogous to soft tissue.</p><p><strong>Results: </strong>Soft tissue thickness increased significantly post-inoculation at 1 and 2 mm below the free gingival margin for both genders and all observed teeth. The significance lasted for weeks 2, 4 and 6, except for female M1s (4 weeks). Color flow velocity was significantly higher compared with baseline for 6 weeks, except for male PM4 (2 weeks). Color flow power did not show significance for PM3 and 4, only in M1 (except male week 4). Significance also extended to tooth type and sex.</p><p><strong>Conclusion: </strong>Periodontal tissue dimension and color flow velocity increased in correlation to bacterial inoculation. Further studies are needed to obtain an understanding of the underlying biology observed here. Eruption of dentition may have been a confounding factor for inflammation interpretation.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasound in Medicine and Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.014","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ACOUSTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Current diagnostic methods of inflammatory periodontal diseases, e.g., visual evaluation, periodontal probing, and radiographs, are either subjective or insensitive. Intra-oral high-frequency ultrasound was investigated to quantify inflammation by detecting tissue dimensional and perfusion changes.
Methods: A cohort of 15-month-old mini-pigs, 4 female/male each, was analyzed. Pre-molars (PM) 3 and 4, as well as first molars (M1), were scanned. In bi-weekly time intervals all 4 quadrants were randomly enrolled and bacterial injection followed each quadrant scan in a weekly fashion. Soft tissue dimensions were obtained from B-mode images and statistically analyzed to identify correlations to inoculation time, i.e., response to bacterial loading, tooth type and sex, using analysis of variance and regression analysis. Color flow velocity and power-weighted color pixel density was obtained and statistically analyzed analogous to soft tissue.
Results: Soft tissue thickness increased significantly post-inoculation at 1 and 2 mm below the free gingival margin for both genders and all observed teeth. The significance lasted for weeks 2, 4 and 6, except for female M1s (4 weeks). Color flow velocity was significantly higher compared with baseline for 6 weeks, except for male PM4 (2 weeks). Color flow power did not show significance for PM3 and 4, only in M1 (except male week 4). Significance also extended to tooth type and sex.
Conclusion: Periodontal tissue dimension and color flow velocity increased in correlation to bacterial inoculation. Further studies are needed to obtain an understanding of the underlying biology observed here. Eruption of dentition may have been a confounding factor for inflammation interpretation.
期刊介绍：
Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
