Potential of phosphatase and tensin gene polymorphisms as salivary biomarkers in oral squamous cell carcinoma - A cross-sectional study.

Q3 Medicine

Journal of Oral and Maxillofacial Pathology Pub Date : 2024-10-01 Epub Date: 2024-12-31 DOI:10.4103/jomfp.jomfp_118_24

Bindiya R Narang, Revati S Deshmukh, Sangeeta J Palaskar, Anirudha Bartake, Swati Patil, Priyanka Nitin

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引用次数: 0

Abstract

Background: PTEN (phosphatase and tensin homologue) is a tumour suppressor gene which is well known for its negative regulation on phosphatidylinositol 3-kinase (PI3K) pathway, thereby controlling the cellular growth and proliferation in the process of carcinogenesis. In the present study, the frequency of the genotypes of the PTEN gene, (rs2943773, rs1234224, rs9651495, rs3827678, and rs11202600) has been observed in individuals with oral squamous cell carcinoma (OSCC) using real-time polymerase chain reaction (RTPCR).

Methodology: Saliva samples were collected from healthy individuals and individuals with OSCC. DNA extraction was done, followed by PCR using fluidigmn technique to observe the frequency of the genotypes.

Results: Variation was observed in the distribution of frequencies of the alleles (rs9651495 and rs1234224) of the PTEN gene between the healthy individual and those with occurrence of OSCC. The other genotypes did not show any statistically significant difference in the distribution between the study group and the control group, nor any association was observed with OSCC.

Conclusion: The variation in the frequency of rs9651495 and rs1234224 of the PTEN gene suggests an association between the "PTEN" gene and "OSCC" and hence its use in the panel of diagnostic markers. This also opens the field for future research in the therapeutic applications of the PTEN gene.

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磷酸酶和紧张素基因多态性作为口腔鳞状细胞癌唾液生物标志物的潜力-一项横断面研究。

背景：PTEN （phosphatase and tensin homologue）是一种肿瘤抑制基因，其负调控磷脂酰肌醇3-激酶（PI3K）通路，从而在癌变过程中控制细胞生长和增殖。本研究采用实时聚合酶链式反应（RTPCR）技术，观察了口腔鳞癌（OSCC）患者中PTEN基因（rs2943773、rs1234224、rs9651495、rs3827678和rs11202600）基因型的频率。方法：收集健康人和OSCC患者的唾液样本。提取DNA，采用荧光定量PCR技术观察基因型出现频率。结果：PTEN基因等位基因rs9651495和rs1234224在健康人与OSCC患者之间的频率分布存在差异。其他基因型在研究组和对照组之间的分布没有统计学上的显著差异，也没有观察到与OSCC的关联。结论：PTEN基因rs9651495和rs1234224的频率变化提示“PTEN”基因与“OSCC”存在关联，可用于诊断标记。这也为PTEN基因的治疗应用开辟了未来的研究领域。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral and Maxillofacial Pathology Medicine-Otorhinolaryngology

CiteScore

1.40

自引率

0.00%

发文量

115

期刊介绍： The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.