Possible therapeutic effects of PROK2 in testicular ischemia/reperfusion injury: A preclinical study in Wistar albino rats.

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-02-08 DOI:10.1016/j.tice.2025.102778

Ilayda Uysal, Nesibe Yilmaz, Semir Gul, Kevser Tanbek, Umit Yilmaz

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引用次数: 0

Abstract

This study aimed to investigate the therapeutic effects of PROK2 on oxidative stress, inflammatory response, and tissue damage caused by ischaemia/reperfusion (I/R) in testicular tissue. A total of 48 prepubertal male Wistar albino rats were divided into four groups: sham, testicular torsion/detorsion (TTD), testicular torsion + PROK2 + detorsion (TT + PROK2 + TD), and testicular torsion/detorsion + PROK2 (TTD + PROK2) (n = 12). Testicular torsion/detorsion surgeries (2 hours of torsion followed by 24 hours of detorsion) were performed on all groups except the sham group. The TT + PROK2 + TD group received a single dose of PROK2 (60 nmol/kg) intraperitoneally 30 minutes before the end of torsion, while the TTD + PROK2 group received a single dose of PROK2 (60 nmol/kg) at the beginning of detorsion. Subsequently, biochemical parameters (serum testosterone level, total antioxidant capacity (TAS), total oxidant capacity (TOS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels in testicular tissue), as well as histopathological and immunohistochemical changes, were evaluated. In TTD, a significant decrease was observed in testosterone and TAS levels, while there was an increase in TOS, TNF-α, and IL-6 levels. PROK2 treatment reduced inflammatory parameters and enhanced antioxidant parameters and testosterone levels. Additionally, a low Johnsen's score and spermatogonia count in the TTD group improved with PROK2 treatment. We concluded that PROK2, by exhibiting antioxidative and anti-inflammatory properties, may have a therapeutic effect on I/R-induced tissue damage in testicular tissue.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.