The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve.

IF 2.6 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Sciences Pub Date : 2025-04-01 Epub Date: 2025-02-13 DOI:10.1007/s43032-025-01811-z

Qianwen Ma, Lifei Wu, Jianfei Wu, Binfei Ni, Jiajia Wang, Shiyan Song

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引用次数: 0

Abstract

Objective: To investigate the mechanism in the progression of decreased ovarian reserve (DOR).

Methods: Three-month-old female SD rats were employed and randomly divided into the model group and the normal group. The model group was intraperitoneally injected with 4-vinylcyclohexene diepoxide (VCD). Thereafter, blood sample from the abdominal aorta was taken, and rats were sacrificed, and ovarian tissues were obtained by laparotomy.

Results: HE staining results revealed that the model group exhibited significantly reduced ovarian volume, increased follicular atresia, and decreased quantities of growing follicles and corpus luteum, thereby indicating degraded reserve function of ovarian. TEM images revealed that prominent autophagic vacuoles could be observed in the model group, accompanied by the mitochondria shrinkage and generation of the autophagosome. The expression of Pink1, Parkin, BNIP3L and LC3II genes in ovaries of the model group was significantly higher than those of the normal group (P < 0.05). In addition, the protein expression of Pink1, Parkin, BNIP3L and LC3II in ovaries of the model group were higher than those of the normal group (P < 0.05). The expression of Fe²⁺ and GSH in oocytes of the model group was higher than those of the normal group (P < 0.05). The expression of FTH1 and GPX4 in oocytes of the model group was significantly higher than those of the normal group (P < 0.05).

Conclusions: Mitochondrial autophagy and ferroptosis may participate in the progression of decreased ovarian reserve (DOR).

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来源期刊

Reproductive Sciences 医学-妇产科学

CiteScore

5.50

自引率

3.40%

发文量

322

审稿时长

4-8 weeks

期刊介绍： Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.