A Model of Combination Therapy of Lewis Lung Carcinoma using Resection and Cytostatics.

IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
N N Ermakova, E G Skurikhin, I L Zharkikh, M A Zhukova, V Yu Pan, E S Pan, M Yu Minakova, S G Morozov, A A Kubatiev, A M Dygai
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引用次数: 0

Abstract

We propose a model of combination treatment of Lewis lung carcinoma (LLC) in C57BL/6 mice that includes tumor resection and chemotherapy. A single injection of 5×106 LLC cells into the right lateral subcostal region caused the growth of the primary tumor and its metastasis to the lung. For reducing metastasis and mortality after resection, the primary tumor should be removed with subcutaneous fat on day 8 after inoculation. Antitumor and antimetastatic effects and reduced mortality were achieved by intraperitoneal injection of carboplatin (63.3 mg/kg) and paclitaxel (13.3 mg/kg); chemotherapy was administered twice. The combination of the two approaches increased the survival: the antitumor and antimetastatic effects were observed in 60% of mice with LLC relative to resection alone or treatment with cytostatics alone. However, chemoresistance was formed in 40% of mice, the values of the tumor growth inhibition index and the metastasis inhibition index decreased. This was accompanied by the appearance of cancer stem cells in the circulation with the potential for the formation of spheroids in vitro. The presented model (combination of tumor resection and chemotherapy) can be used in the development of new approaches to improve treatment efficiency in patients with lung cancer.

Lewis肺癌切除加细胞抑制剂联合治疗的模型。
我们提出了一种包括肿瘤切除和化疗的C57BL/6小鼠Lewis肺癌(LLC)联合治疗模型。将5×106 LLC细胞单次注射到右侧肋下区域,导致原发肿瘤生长并转移到肺。为减少肿瘤转移和术后死亡率,应在接种后第8天用皮下脂肪切除原发肿瘤。腹腔注射卡铂(63.3 mg/kg)和紫杉醇(13.3 mg/kg)可达到抗肿瘤和抗转移作用,降低死亡率;化疗两次。两种方法的结合提高了生存率:相对于单独切除或单独使用细胞抑制剂治疗,60%的LLC小鼠观察到抗肿瘤和抗转移作用。但40%的小鼠产生耐药,肿瘤生长抑制指数和转移抑制指数下降。与此同时,在体外循环中出现了可能形成球状体的癌症干细胞。所提出的模型(肿瘤切除和化疗联合)可用于开发新的方法来提高肺癌患者的治疗效率。
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来源期刊
Bulletin of Experimental Biology and Medicine
Bulletin of Experimental Biology and Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
14.30%
发文量
265
审稿时长
2 months
期刊介绍: Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.
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