Occurrence, Risk Factors, and Antibiotic Resistance Patterns of Salmonella Isolates Obtained From Dairy Farms in Gondar Town, Northwest Ethiopia.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2025-01-28 eCollection Date: 2025-01-01 DOI:10.1155/bmri/7778947
Tsedalu Yirsa, Alene Tigistu
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引用次数: 0

Abstract

Background: Salmonellosis is a foodborne diarrheal disease with significant public health and economic implications for humans and animals. Nevertheless, its epidemiological effects and multidrug resistance remain poorly studied in the study area. Methods: A cross-sectional study was conducted from March to August 2022 to determine the occurrence, risk factors, and antibiotic susceptibility patterns of Salmonella isolates in the study dairy farms. A total of 232 samples (200 study animals and 32 bulk tank milk) were collected from purposively selected dairy farms in the study area. Again, fecal and bulk tank milk samples were collected, pre-enriched, and cultured to examine the presence of Salmonella following standard techniques. The pure 30 isolates were also subjected to a Kirby-Bauer disk diffusion test on Muller-Hinton agar to assess their antibiotic susceptibility patterns. Binary logistic regression analysis in Statistical Package for Social Sciences (SPSS) Version 25 was used to determine the strength of the risk factors associated with the occurrence of this disease. Findings: The overall prevalence of Salmonella isolates was 12.9% (30/232) across all samples. Among these, 11.5% (23/200) of the fecal samples from the study animals and 21.9% (7/32) of the bulk tank milk samples tested positive for this disease. A statistically significant association was observed between the presence of the Salmonella isolates and the age of the study animals, farm size, bedding, and udder washing practices. The isolates also showed the highest resistance to ampicillin (100%) and tetracycline (96.7%). Conversely, the isolates were most sensitive to ciprofloxacin (100%) and kanamycin (90%). Conclusions: Generally, the high presence of Salmonella isolates and its resistance to drugs pose significant economic and public health challenges. Thus, it is crucial to implement improved management practices and appropriate antibiotic therapies in the study dairy farms.

埃塞俄比亚西北部贡达尔镇奶牛场沙门氏菌分离株的发生、危险因素和抗生素耐药性模式
背景:沙门氏菌病是一种食源性腹泻疾病,对人类和动物具有重大的公共卫生和经济影响。然而,其流行病学影响和多药耐药性在研究地区的研究仍然很少。方法:于2022年3月至8月进行横断面研究,以确定研究奶牛场沙门氏菌分离株的发生情况、危险因素和抗生素敏感性。共有232份样本(200只研究动物和32只散装罐奶)从研究区有目的地选择的奶牛场收集。再次,收集粪便和散装罐乳样品,预富集,培养,以检查沙门氏菌的存在,按照标准技术。对30株纯菌株进行了Kirby-Bauer圆盘扩散试验,以评估其抗生素敏感性。使用社会科学统计软件包(SPSS)第25版中的二元逻辑回归分析来确定与该疾病发生相关的危险因素的强度。结果:所有样本中分离沙门氏菌的总体流行率为12.9%(30/232)。其中,11.5%(23/200)的研究动物粪便样本和21.9%(7/32)的散装罐乳样本检测出此病阳性。观察到沙门氏菌分离株的存在与研究动物的年龄、农场规模、床上用品和洗乳习惯之间存在统计学上显著的关联。对氨苄西林(100%)和四环素(96.7%)的耐药性最高。相反,分离株对环丙沙星(100%)和卡那霉素(90%)最敏感。结论:总体而言,分离沙门氏菌的高存在率及其耐药性构成了重大的经济和公共卫生挑战。因此,在研究的奶牛场实施改进的管理实践和适当的抗生素治疗是至关重要的。
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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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