Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss.

IF 6.4 1区 医学 Q1 CELL & TISSUE ENGINEERING
Ricardo Whitaker, Samuel Sung, Tina Tylek, Gregory E Risser, Erin M O'Brien, Phoebe Ellin Chua, Thomas Li, Ryan J Petrie, Lin Han, Benjamin I Binder-Markey, Kara L Spiller
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引用次数: 0

Abstract

We took a systems approach to the analysis of macrophage phenotype in regenerative and fibrotic volumetric muscle loss outcomes in mice together with analysis of systemic inflammation and of other leukocytes in the muscle, spleen, and bone marrow. Differences in expression of macrophage phenotype markers occurred as early as day 1, persisted to at least day 28, and were associated with increased numbers of leukocytes in the muscle and bone marrow, increased pro-inflammatory marker expression in splenic macrophages, and changes in the levels of pro-inflammatory cytokines in the blood. The most prominent differences were in muscle neutrophils, which were much more abundant in fibrotic outcomes compared to regenerative outcomes at day 1 after injury. However, neutrophil depletion had little to no effect on macrophage phenotype or on muscle repair outcomes. Together, these results suggest that the entire system of immune cell interactions must be considered to improve muscle repair outcomes.

损伤大小对体积性肌肉损失中局部和全身免疫细胞动力学的影响。
我们采用系统方法分析小鼠再生和纤维化体积性肌肉损失结果中的巨噬细胞表型,并分析全身炎症和肌肉、脾脏和骨髓中的其他白细胞。巨噬细胞表型标志物的表达差异早在第1天就出现了,并持续到至少28天,并且与肌肉和骨髓中白细胞数量增加、脾巨噬细胞中促炎标志物表达增加以及血液中促炎细胞因子水平的变化有关。最显著的差异是肌肉中性粒细胞,与损伤后第1天的再生结果相比,纤维化结果中中性粒细胞要丰富得多。然而,中性粒细胞耗竭对巨噬细胞表型或肌肉修复结果几乎没有影响。总之,这些结果表明,必须考虑整个免疫细胞相互作用系统来改善肌肉修复结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
npj Regenerative Medicine
npj Regenerative Medicine Engineering-Biomedical Engineering
CiteScore
10.00
自引率
1.40%
发文量
71
审稿时长
12 weeks
期刊介绍: Regenerative Medicine, an innovative online-only journal, aims to advance research in the field of repairing and regenerating damaged tissues and organs within the human body. As a part of the prestigious Nature Partner Journals series and in partnership with ARMI, this high-quality, open access journal serves as a platform for scientists to explore effective therapies that harness the body's natural regenerative capabilities. With a focus on understanding the fundamental mechanisms of tissue damage and regeneration, npj Regenerative Medicine actively encourages studies that bridge the gap between basic research and clinical tissue repair strategies.
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