The recombination landscape of introgression in yeast.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-02-12 eCollection Date: 2025-02-01 DOI:10.1371/journal.pgen.1011585
Enrique J Schwarzkopf, Nathan Brandt, Caiti Smukowski Heil
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引用次数: 0

Abstract

Meiotic recombination is an evolutionary force that acts by breaking up genomic linkage, increasing the efficacy of selection. Recombination is initiated with a double-strand break which is resolved via a crossover, which involves the reciprocal exchange of genetic material between homologous chromosomes, or a non-crossover, which results in small tracts of non-reciprocal exchange of genetic material. Crossover and non-crossover rates vary between species, populations, individuals, and across the genome. In recent years, recombination rate has been associated with the distribution of ancestry derived from past interspecific hybridization (introgression) in a variety of species. We explore this interaction of recombination and introgression by sequencing spores and detecting crossovers and non-crossovers from two crosses of the yeast Saccharomyces uvarum. One cross is between strains which each contain introgression from their sister species, S. eubayanus, while the other cross has no introgression present. We find that the recombination landscape is significantly different between S. uvarum crosses, and that some of these differences can be explained by the presence of introgression in one cross. Crossovers are significantly reduced in heterozygous introgression compared to syntenic regions in the cross without introgression. This translates to reduced allele shuffling within introgressed regions, and an overall reduction of shuffling on most chromosomes with introgression compared to the syntenic regions and chromosomes without introgression. Our results suggest that hybridization can significantly influence the recombination landscape, and that the reduction in allele shuffling contributes to the initial purging of introgression in the generations following a hybridization event.

酵母中基因渗入的重组景观。
减数分裂重组是一种进化力量,它通过破坏基因组联系,增加选择的效力。重组开始于双链断裂,通过交叉解决,这涉及同源染色体之间的遗传物质的互惠交换,或非交叉,这导致小束的遗传物质的非互惠交换。交叉和非交叉率在物种、种群、个体和整个基因组之间有所不同。近年来,重组率与多种物种过去种间杂交(渐渗)衍生的祖先分布有关。我们通过测序孢子和检测两个杂交酵母的杂交和非杂交来探索这种重组和渗入的相互作用。一个杂交是在两个菌株之间进行的,其中每个菌株都含有来自其姐妹种的基因渗入,而另一个杂交则没有基因渗入。研究发现,不同杂交组合的重组景观存在显著差异,其中一些差异可以用一个杂交组合的基因渗入来解释。杂合渐渗的杂交明显低于无渐渗的共合区。这意味着在渐渗区域内的等位基因洗牌减少,并且与非渐渗区域和染色体相比,大多数具有渐渗的染色体上的洗牌总体减少。我们的研究结果表明,杂交可以显著影响重组格局,并且等位基因洗牌的减少有助于杂交事件发生后世代中基因渗入的初始清除。
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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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