From eggs to guts: Symbiotic association of Sodalis nezarae sp. nov. with the Southern green shield bug Nezara viridula.

IF 3.5 3区生物学 Q2 MICROBIOLOGY

FEMS microbiology ecology Pub Date : 2025-02-20 DOI:10.1093/femsec/fiaf017

Magda A Rogowska-van der Molen, Alejandro Manzano-Marín, Jelle L Postma, Silvia Coolen, Theo van Alen, Robert S Jansen, Cornelia U Welte

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引用次数: 0

Abstract

Phytophagous insects engage in symbiotic relationships with bacteria that contribute to digestion, nutrient supplementation, and development of the host. The analysis of shield bug microbiomes has been mainly focused on the gut intestinal tract predominantly colonized by Pantoea symbionts and other microbial community members in the gut or other organs have hardly been investigated. In this study, we reveal that the Southern green shield bug Nezara viridula harbours a Sodalis symbiont in several organs, with a notable prevalence in salivary glands, and anterior regions of the midgut. Removing external egg microbiota via sterilization profoundly impacted insect viability but did not disrupt the vertical transmission of Sodalis and Pantoea symbionts. Based on the dominance of Sodalis in testes, we deduce that N. viridula males could be involved in symbiont vertical transmission. Genomic analyses comparing Sodalis species revealed that Sodalis sp. Nvir shares characteristics with both free-living and obligate insect-associated Sodalis spp. Sodalis sp. Nvir also displays genome instability typical of endosymbiont lineages, which suggests ongoing speciation to an obligate endosymbiont. Together, our study reveals that shield bugs harbour unrecognized symbionts that might be paternally transmitted.

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从卵到内脏：nezarae sp. 11与南方绿盾虫Nezara viridula的共生关系。

植食昆虫与细菌的共生关系有助于消化、营养补充和宿主的发育。盾菌菌群的分析主要集中在以Pantoea共生体为主的肠道，而对肠道或其他器官中的其他微生物群落成员的研究很少。在这项研究中，我们发现南方绿盾虫Nezara viridula在几个器官中都有一个Sodalis共生体，在唾液腺和中肠前区有明显的流行。通过灭菌去除卵外微生物群会严重影响昆虫的生存能力，但不会破坏Sodalis和Pantoea共生体的垂直传播。基于该菌在睾丸中的优势地位，我们推断病毒乳杆菌可能参与了共生垂直传播。基因组分析表明，Sodalis sp. Nvir与自由生活和专性昆虫相关的Sodalis spp具有相同的特征，Sodalis sp. Nvir也表现出典型的内共生谱系的基因组不稳定性，这表明物种正在向专性内共生进化。总之，我们的研究表明，盾虫窝藏着未被识别的共生体，这些共生体可能是父系传播的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FEMS microbiology ecology 生物-微生物学

CiteScore

7.50

自引率

2.40%

发文量

132

审稿时长

3 months

期刊介绍： FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms