Enhancement of Semen Cryopreservation from Native Thai Bulls Through Moringa oleifera Leaf Extract Supplementation.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2025-02-05 DOI:10.3390/ani15030439

Supakorn Authaida, Wuttigrai Boonkum, Vibuntita Chankitisakul

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引用次数: 0

Abstract

Cryopreservation of native Thai bull semen often results in significant post-thaw quality reduction, underscoring the need for effective cryoprotective strategies. This study investigated the effect of Moringa oleifera leaf extract (MOLE) as an antioxidant supplementation by incorporating four MOLE concentrations (0-1.5% [w/v]) into a standard semen extender, followed by cryopreservation using liquid nitrogen vapor freezing. Data were analyzed using a randomized complete block design with Tukey's post hoc test (p < 0.05). Post-thaw analysis of semen revealed that 1 mg/mL MOLE significantly enhanced total sperm motility, progressive sperm motility, sperm viability, and sperm plasma membrane integrity compared to the control and other MOLE concentrations (p < 0.05). This concentration also improved the amplitude of lateral head displacement and curvilinear velocity and reduced malondialdehyde levels in semen samples (p < 0.05), indicating reduced lipid peroxidation. Higher MOLE concentrations negatively impacted semen quality. In conclusion, supplementation with 1 mg/mL MOLE markedly improved post-thaw semen quality and reduced lipid peroxidation, suggesting its potential as an antioxidant for enhancing reproductive outcomes in native Thai bulls.

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).