Poloxamer 407-Based Hydrogels Containing Rutin Increase the In Vitro and In Vivo Wound Healing Phenomena.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-03-17 Epub Date: 2025-02-12 DOI:10.1021/acsabm.4c01452

Elena Giuliano, Agnese Gagliardi, Anam Farhan, Silvia Voci, Nicola Costa, Stefania Bulotta, Vincenzo Mollace, Ernesto Palma, Muhammad Majid, Donato Cosco

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Abstract

Rutin is a flavonoid glycoside found in many plants, known for its various pharmacological properties. However, its therapeutic potential is limited by its low water solubility, which restricts its bioavailability. Poloxamer 407 (P407) is a multifunctional excipient, which has proven effective in delivering poorly water-soluble drugs and promoting the healing process. Based on this information, this study investigates the wound healing potential of rutin-loaded P407 hydrogels, as an innovative pharmaceutical formulation. In vitro scratch assays on human keratinocytes revealed that combining rutin with P407 significantly enhances cell proliferation as compared to the single components. Moreover, the proposed hydrogel improved wound healing more effectively in rats than the free drug or the commercial wound dressing DuoDERM. Histological analyses confirmed that the formulation increased epithelialization and collagen synthesis in injured epidermises. These findings provide a strong rationale for the use of rutin-loaded P407 hydrogels for the treatment of skin injuries.

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含芦丁的波洛沙姆407水凝胶可促进体内和体外伤口愈合。

芦丁是一种在许多植物中发现的类黄酮苷，以其各种药理特性而闻名。然而，其治疗潜力受到其低水溶性的限制，这限制了其生物利用度。poloxam407 （P407）是一种多功能赋形剂，已被证明对输送水溶性较差的药物和促进愈合过程有效。基于这些信息，本研究探讨了芦丁负载P407水凝胶作为一种创新的药物配方的伤口愈合潜力。体外对人角质形成细胞的刮擦实验显示，与单独使用相比，芦丁与P407联合使用可显著增强细胞增殖能力。此外，所提出的水凝胶比游离药物或商业伤口敷料更有效地促进大鼠伤口愈合。组织学分析证实，该配方增加了受伤表皮的上皮化和胶原合成。这些发现为使用负载芦丁的P407水凝胶治疗皮肤损伤提供了强有力的理论依据。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.