Antimicrobial and antioxidant potential of Yemeni Sidr honey against multidrug-resistant pathogens – in vitro and in silico studies

IF 3.4 Q2 PHARMACOLOGY & PHARMACY
Hani A. Alhadrami, Ahmed M. Sayed, Hossam M. Hassan, Mostafa E. Rateb, Marwa A. Taher
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引用次数: 0

Abstract

Background

Honey’s medical values have been extensively recorded in literature. Yemeni Sidr honey was reported to treat many ailments like stomach and respiratory disorders. ESKAPE and other multidrug-resistant pathogens are considered one of the top three risks to global public health, so alternative strategies become critical demand against such pathogens or their biofilms. The current study aimed to explore the antibacterial and antioxidant potential of the Yemeni Sidr honey extracts. The antibacterial activity of the two Yemeni Sidr honey extracts (ST and SM) was assessed against different pathogenic strains. The antioxidant activity was also evaluated using ORAC, ABST, 5-LOX, and DPPH. Furthermore, 2D HSQC data of both ST and SM honey extracts were collected uploaded to the SMART platform to identify the possible metabolites in these extracts. The identified metabolites were analyzed using docking and molecular dynamic simulations (MDS) to identify the key players in the antibacterial action.

Results

The antibacterial activity revealed that ST and SM extracts have similar activity against all tested pathogens. ST extract exhibited superior antibiofilm effect against P. aeruginosa and C. albicans by 68.2% and 62.6%, respectively, exceeding the reference standards. Moreover, ST extract displayed the highest antioxidant power against all assays except the DPPH assay. SMART dereplication of the HSQC data of ST extract revealed the annotations of five carbohydrates (fructose, glucose, mannose, maltose, and sucrose); while, SM extract showed three major phenolic compounds (chrysin, ellagic acid, and caffeic acid), in which chrysin and ellagic acid were likely the key players in the antibacterial action, based on MDS.

Conclusions

The study confirmed the effectiveness of Sidr honey against the tested multidrug-resistant pathogens. Additionally, our observations shed the light on the main secondary constituents in Yemini Sidr honey extracts, and their effective role in multidrug-resistant pathogens growth inhibition.

也门Sidr蜂蜜抗多药耐药病原体的抗菌和抗氧化潜力-体外和计算机研究
蜂蜜的医学价值在文献中有广泛的记载。据报道,也门Sidr蜂蜜可以治疗胃病和呼吸系统疾病等多种疾病。ESKAPE和其他耐多药病原体被认为是全球公共卫生的三大风险之一,因此,针对此类病原体或其生物膜的替代战略成为至关重要的需求。本研究旨在探索也门Sidr蜂蜜提取物的抗菌和抗氧化潜力。研究了两种也门Sidr蜂蜜提取物(ST和SM)对不同病原菌的抑菌活性。用ORAC、ABST、5-LOX和DPPH评价其抗氧化活性。此外,收集ST和SM蜂蜜提取物的二维HSQC数据上传到SMART平台,以鉴定这些提取物中可能的代谢物。利用对接和分子动力学模拟(MDS)对鉴定的代谢物进行分析,以确定抗菌作用的关键参与者。结果两种提取物对病原菌的抑菌活性相近。ST提取物对铜绿假单胞菌和白色假单胞菌的抗菌效果分别为68.2%和62.6%,均超过参考标准。此外,ST提取物对除DPPH外的所有实验均显示出最高的抗氧化能力。对ST提取物的HSQC数据进行SMART反复制,发现5种碳水化合物(果糖、葡萄糖、甘露糖、麦芽糖和蔗糖)的注释;而SM提取物中含有三种主要的酚类化合物(金菊素、鞣花酸和咖啡酸),基于MDS,其中金菊素和鞣花酸可能是抗菌作用的关键成分。结论本研究证实了甘露蜂蜜对多药耐药病原菌的抑制作用。此外,我们的观察揭示了叶米尼蜂蜜提取物的主要次级成分,以及它们在抑制多药耐药病原体生长中的有效作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
0.00%
发文量
44
审稿时长
23 weeks
期刊介绍: Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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