Report of Anaplasma marginale in Mithun (Bos frontalis) from North Eastern Hilly Region of India

IF 1.2 3区 农林科学 Q4 PARASITOLOGY
Jayanta Kumar Chamuah, Siju Susan Jacob, Angughali Aheto Sumi, Livika T. Awomi, Lipenthang Ezung, Mahak Singh, S. Girish Patil
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引用次数: 0

Abstract

Purpose

To investigate the seroprevalence and molecular characterization of Anaplasma parasites in mithun (Bos frontalis) and their tick vectors in Nagaland and Arunachal Pradesh, India using serological and molecular methods.

Methods

Mithun sera were screened for Anaplasma antibodies using a competitive ELISA targeting the MSP5 antigen. Blood samples and ticks collected from mithun were analyzed using PCR targeting the RNA polymerase subunit beta (rpoB) gene for Anaplasma species detection, followed by sequencing and phylogenetic analysis of positive samples.

Results

Serological testing detected Anaplasma antibodies in 11.90% (5/42) of tested mithun. PCR analysis of 358 mithun blood samples identified eight animals (2.23%) positive for A. marginale, with identity confirmed through sequencing and phylogenetic analysis. Among the collected ticks, Anaplasma was detected in one R. microplus specimen (1/340), while all Amblyomma spp. (n = 25) tested negative. Phylogenetic analysis of the rpoB gene sequences showed the mithun isolates clustering with A. marginale reference sequences from diverse regions.

Conclusion

This study provides the first documented evidence of A. marginale infection in mithun populations and associated ticks in Northeast India, supported by molecular, serological and phylogenetic analyses. The findings underscore the importance of multi-faceted diagnostic approaches, targeted vector control, and ongoing surveillance to address regional disease risks.

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来源期刊
Acta Parasitologica
Acta Parasitologica 医学-寄生虫学
CiteScore
3.10
自引率
6.70%
发文量
149
审稿时长
6-12 weeks
期刊介绍: Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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