Electrochemical biosensor based on strand displacement reaction for on-site detection of Skeletonema costatum

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-02-13 DOI:10.1007/s00604-025-06966-9

Yaling Liu, Yibo Zhang, Changrui Ye, Yayi He, Shaopeng Wang, Man Zhang, Hao Fu, Shan Lu, Liwei Wang

{"title":"Electrochemical biosensor based on strand displacement reaction for on-site detection of Skeletonema costatum","authors":"Yaling Liu, Yibo Zhang, Changrui Ye, Yayi He, Shaopeng Wang, Man Zhang, Hao Fu, Shan Lu, Liwei Wang","doi":"10.1007/s00604-025-06966-9","DOIUrl":null,"url":null,"abstract":"<div><p> An electrochemical biosensor was constructed for quantitatively detecting <i>Skeletonema costatum</i> (<i>S. costatum</i>) by combining the electrode modification material (NC-Au) with a strand displacement reaction (SDR). The SDR process addresses the issues of steric hindrance and electrostatic repulsion resulting from the large size of genomic DNA. It enhances the efficiency of the interfacial hybridization reaction and endows the biosensor with remarkable sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) were 33.43 fg/μL (831 cells/L) and 87.21 fg/μL (2112 cells/L), respectively. Additionally, the biosensor has demonstrated excellent accuracy compared to methods such as microscopy and ddPCR (<i>P</i> > 0.05). Subsequent assessment of the adjacent waters of the Beibu Gulf using biosensors indicated a low risk of <i>S. costatum</i> red tide outbreaks in the region, which is consistent with the findings of the ecological survey. Therefore, we believe that this biosensor can provide a completely new idea for the dynamic monitoring and early warning of <i>S. costatum</i> red tide.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-06966-9","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

An electrochemical biosensor was constructed for quantitatively detecting Skeletonema costatum (S. costatum) by combining the electrode modification material (NC-Au) with a strand displacement reaction (SDR). The SDR process addresses the issues of steric hindrance and electrostatic repulsion resulting from the large size of genomic DNA. It enhances the efficiency of the interfacial hybridization reaction and endows the biosensor with remarkable sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) were 33.43 fg/μL (831 cells/L) and 87.21 fg/μL (2112 cells/L), respectively. Additionally, the biosensor has demonstrated excellent accuracy compared to methods such as microscopy and ddPCR (P > 0.05). Subsequent assessment of the adjacent waters of the Beibu Gulf using biosensors indicated a low risk of S. costatum red tide outbreaks in the region, which is consistent with the findings of the ecological survey. Therefore, we believe that this biosensor can provide a completely new idea for the dynamic monitoring and early warning of S. costatum red tide.

Graphical abstract

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.