Carboxyl-converting titration versus other methods for the uronic acid content analysis of alginate oligosaccharide

IF 2.4 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Meng Shao , Yue Yang , Hao Liu , Hui Zhou , Yuanyuan Sun , Xia Zhao , Guangli Yu , Youjing Lv , Quancai Li
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Abstract

This study presents a carboxyl-conversing titration method for determining the uronic acid content of alginate oligosaccharide (AOS). All C6 carboxyl groups on the monosaccharide residues of AOS were initially converted into carboxylates, which were subsequently transformed into carbonates through carbonization and incineration. Quantification was finally done through acid titration. The AOS content, which was represented by the amount of monosaccharide residues (ManA and GulA residues) in AOS sample, was calculated based on the stoichiometric relationship between the C6 carboxyl group and monosaccharide residue. To evaluate its performance, the carboxyl-conversing titration method was compared with the sulfuric acid-carbazole method and 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization high performance liquid chromatography (PMP-HPLC) method. The content of the uronic acid in AOS sample were detected 96.24 % ± 0.42 % using the carboxyl-conversing titration method, 186.85 ± 0.49 % (ManA/GulA = 1/1 as the reference standard) using the sulfuric acid-carbazole method, and 3.00 ± 0.08 % using the PMP-HPLC method. The carboxyl-conversing titration method demonstrated the highest accuracy of the content value. The validation of this method was also confirmed in the terms of specificity, limits of detection and quantification, recovery, repeatability, intermediate precision and durability. Compared to the sulfuric acid-carbazole and PMP-HPLC methods, the carboxyl-conversing titration method was proved easy to operate, reliable, and independent of reference standards. This innovative method offers a promising solution for accurately determining AOS content in laboratory and industrial settings.

Abstract Image

羧基转换滴定法与其他方法比较分析海藻酸盐低聚糖的糖醛酸含量
研究了用羧基转换滴定法测定海藻酸寡糖(AOS)中醛酸含量的方法。AOS的单糖残基上的所有C6羧基都先转化为羧酸盐,再经过炭化和焚烧转化为碳酸盐。最后通过酸滴定进行定量。AOS含量以AOS样品中单糖残基(ManA和GulA残基)的数量为代表,根据C6羧基与单糖残基的化学计量关系计算AOS含量。为了评价羧基转换滴定法的性能,将其与硫酸-咔唑法和1-苯基-3-甲基-5-吡唑酮(PMP)柱前衍生高效液相色谱(PMP- hplc)法进行了比较。羧基转换滴定法测定AOS样品中脲酸的含量为96.24%±0.42%,硫酸-咔唑法测定为186.85±0.49% (ManA/GulA = 1/1), PMP-HPLC法测定为3.00±0.08%。羧基转换滴定法测定含量值的准确度最高。从特异性、检出限、定量限、回收率、重复性、中间精密度和持久性等方面验证了该方法的有效性。与硫酸-咔唑法和PMP-HPLC法相比,羧基转换滴定法操作简便、可靠、不依赖于标准品。这种创新的方法为在实验室和工业环境中准确测定AOS含量提供了一种有前途的解决方案。
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来源期刊
Carbohydrate Research
Carbohydrate Research 化学-生化与分子生物学
CiteScore
5.00
自引率
3.20%
发文量
183
审稿时长
3.6 weeks
期刊介绍: Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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