Serotype and genotype shift detection over two consecutive periods of dengue virus infection in a tertiary care hospital

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2025-02-14 DOI:10.1016/j.ijmmb.2025.100807

Sandhya Bhat K , Mailan Natarajan , Natarajan Vasanthi , Sudhagar Mookkappan , Bhavadharani Pandian , Shashikala Nair , Reba Kanungo

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引用次数: 0

Abstract

Purpose

Dengue virus infection in humans can also lead to severe complications like dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) estimates around 50 to 100 million dengue infections annually, with approximately 500,000 cases of DHF and 22,000 deaths, predominantly among children. The present study aimed at identifying circulating serotypes of dengue virus in patients by molecular techniques. Serotypes detected were correlated with the clinical profiles, complications, and outcomes in these patients.

Methods

This study was conducted between October 2019 to September 2022. Institute Ethics Committee approval was obtained (Reference no. IEC: RC/18/59). Serum from patients who tested positive for dengue NS1 antigen (NS1Ag) or anti-dengue immunoglobulin M (IgM) were serotyped by dengue reverse transcriptase polymerase chain reaction (RT-PCR). Clinical data was documented in case report forms and transferred to an Excel spreadsheet, and subsequently analyzed to correlate the dengue virus (DENV) serotype with clinical profile and outcome.

Results

The study population included 416 patients, confirmed by Dengue NS1Ag and or anti-Dengue IgM ELISA. Young adults (18–29 yrs) constituted the majority (56 %) of patients. About 85 % of all patients presented with fever, 10.6 % with hemorrhagic fever, and 4.4 % with shock syndrome. Thrombocytopenia with multiple bleeding manifestations was observed in 6.5 % of the patients. The predominant dengue serotype identified was serotype 3 from October 2019 to March 2021, and serotype 2 from April 2021 to September 2022, showing a shift in the serotypes affecting the patients.

Conclusion

Continuous sero- and molecular surveillance plays a crucial role in predicting dengue outbreaks and identifying shifts in circulating serotypes during the early phase of the dengue season. It may also play a role in vaccine preparedness for the control of dengue.

查看原文本刊更多论文

三级保健医院连续两期登革热病毒感染血清型和基因型转移检测

目的：人感染登革病毒还可导致严重并发症，如登革出血热（DHF）和登革休克综合征（DSS）。世界卫生组织（卫生组织）估计，每年约有5 000至1亿登革热感染，约有50万登革出血热病例，22 000人死亡，主要是儿童。本研究旨在通过分子技术鉴定患者中登革热病毒的循环血清型。检测到的血清型与这些患者的临床特征、并发症和结局相关。方法本研究于2019年10月至2022年9月进行。已获得研究所伦理委员会批准(参考文献号：IEC: RC / 18/59)。采用登革热逆转录酶聚合酶链反应（RT-PCR）对登革热NS1抗原（NS1Ag）或抗登革热免疫球蛋白M （IgM）检测阳性的患者进行血清分型。临床数据被记录在病例报告表格中，并转移到Excel电子表格中，随后进行分析，以将登革热病毒（DENV）血清型与临床特征和结果联系起来。结果纳入416例患者，经登革热NS1Ag和/或抗登革热IgM酶联免疫吸附试验证实。青壮年（18-29岁）占大多数（56%）。85%的患者表现为发热，10.6%表现为出血热，4.4%表现为休克综合征。6.5%的患者伴有多种出血表现的血小板减少。2019年10月至2021年3月期间确定的主要登革热血清型为血清3型，2021年4月至2022年9月期间确定的主要登革热血清型为血清2型，表明影响患者的血清型发生了变化。结论持续的血清和分子监测在登革热季节早期预测登革热疫情和确定流行血清型变化方面发挥着至关重要的作用。它还可能在控制登革热的疫苗准备工作中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.