Tenuifilum osseticum sp. nov., a novel thermophilic hydrolytic bacterium within the Tenuifilaceae isolated from a North Ossetian thermal spring, and emended description of the genus Tenuifilum

Abstract

A novel anaerobic moderately thermophilic bacterium, strain 4138-str^T, was isolated from a thermal spring of North Ossetia (Russian Federation). Gram-negative cells were non-sporeforming, straight or curved filamentous rods, occasionally forming rosettes. The strain grew at 30–55 °C, pH range of 6.1–8.7, NaCl range of 0–4 %, with an optimum at 50 °C, pH 7.1–7.5 and 0.2–0.4 % NaCl. It was a chemoorganoheterotroph, growing on simple sugars (glucose, maltose, cellobiose, etc.) and carbohydrates (starch, pullulan, laminarin, xylan, lichenan, curdlan, pachyman) or proteinaceous substrates (peptone, tryptone, gelatin, casein). Sulfur was used as electron acceptor. Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C_15:0 and anteiso-C_15:0. The quinone was MK-7. The size of the whole genome of strain 4138-str^T was 3.275 Mbp; DNA G + C content was 42.1 %. Genome analysis allowed to identify genes encoding carbohydrate-active enzymes and extracellular proteases. In addition, central metabolism and fermentation pathways of strain 4138-str^T were reconstructed. According to both phylogenetic analyses, based on 16S rRNA gene sequences and conserved protein sequences, as well as genome-based comparisons, strain 4138-str^T formed a species-level lineage within Tenuifilum genus of Tenuifilaceae family (phylum Bacteroidota). Here we propose a novel species Tenuifilum osseticum sp. nov. with type strain 4138-str^T(=KCTC 25386^T = VKM B-3628^T = UQM 41477^T).