The outer membrane vesicles of Vibrio parahaemolyticus elicit innate immune responses in mud crab (Scylla paramamosain)

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-02-11 DOI:10.1016/j.aquaculture.2025.742289

Qian Sun , Linying Yun , Kaichun Hu , Han Li , Pengfei Li , Yiyuan Lu , Runwu Gao , Ming Zhang , Ngoc Tuan Tran , Xiuli Chen , Yongzhen Zhao , Yueling Zhang , Shengkang Li

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Abstract

Outer membrane vesicles (OMVs) are extracellular nanostructures secreted by bacteria, which carry various virulence factors and play a pivotal role in the pathogenesis of bacterial infections. In this study, outer membrane vesicles derived from Vibrio parahaemolyticus (Vp-OMVs) were isolated to examine their effects on innate immunity in mud crabs (Scylla paramamosain). Characterization of the Vp-OMVs confirmed the presence of vesicles exhibiting typical OMV morphology and size. Vp-OMVs were internalized by host cells through clathrin-mediated endocytosis, leading to cytotoxicity, increased mortality, tissue vacuolation, and reduced cell viability. Furthermore, Vp-OMVs were found to contain lipopolysaccharide (LPS), which activates the Toll2 receptor, thereby initiating the Toll2 signaling pathway and promoting the expression of antimicrobial peptides. Additionally, internalized Vp-OMVs triggered inflammasome activation and mitochondrial dysfunction, resulting in inflammation and apoptosis. These findings highlight the critical role of Vp-OMVs in the pathogenesis of V. parahaemolyticus infections in mud crabs and offer valuable insights into potential therapeutic strategies for managing these infections.

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.