Effects of selenite on growth and symbiotic nitrogen fixation of soybean

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-02-12 DOI:10.1007/s11104-025-07249-1

Lei Li, Ceng Yi, Zhuqing Zhao, Huan He, Xinwei Liu

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引用次数: 0

Abstract

Aims

Symbiotic nitrogen fixation (SNF) has the potential to enhance the yield of legume crops, particularly under low nitrogen conditions. Selenium (Se) has been demonstrated to enhance plant adaptability to the environment. In this study, the effects of selenite on the growth of soybean and SNF were investigated.

Methods

A hydroponics experiment with four selenite concentrations (0, 0.5, 2, and 10 μM) and a soil experiment with three selenite application rates (0, 2, 5 mg kg⁻¹) were carried out. The soybean variety Zhonghuang 13 and Bradyrhizobium diazoefficiens USDA110 were utilized as experimental materials, Na₂SeO₃ used as the Se source.

Results

The application of Se significantly promoted soybean growth and improved physiological performance. Nodule biomass increased by 36.79% and 32.65% under 10 μM and 5 mg kg⁻¹ Se treatments, respectively, compared to the CK. Nitrogenase activity was markedly improved by 313% under 10 μM Se in hydroponic conditions. Se also elevated nitrogen content in soybean plants, promoting carbohydrate accumulation and transport. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in leaves increased, whereas the malondialdehyde (MDA) content decreased in both roots and leaves following Se application. Metabolomics analysis revealed that Se treatment upregulated amino acid metabolism and the citrate cycle pathways in soybean roots, while downregulating the biosynthesis pathways of flavones, isoflavonoids, and flavonoids.

Conclusions

Selenite promotes soybean and nodule growth while enhancing SNF efficiency. Our results provide a theoretical basis for improving legume production by using Se fertilizer in the context of low nitrogen inputs.

Graphical Abstract

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.