{"title":"Two K2P Channels, TWK-46 and TWK-26 do not affect <i>C. elegans</i> Egg-Laying Behavior.","authors":"Li Chen, Isabel Beets, William Schafer","doi":"10.17912/micropub.biology.001477","DOIUrl":null,"url":null,"abstract":"<p><p>Two-pore domain potassium channels, also known as K2P channels, play vital roles in maintaining the resting membrane potential in excitable cells, affecting a variety of physiological processes across species. The <i>Caenorhabditis elegans</i> ( <i>C. elegans</i> ) genome contains 46 different K2P-encoding genes, yet most of their functions remain unknown. Here, we have investigated the possible roles of two <i>C. elegans</i> K2P channel genes - <i>twk-26</i> and <i>twk-46</i> - that are expressed in the egg-laying neural circuit by characterizing the egg-laying behavior of null mutants generated by CRISPR/Cas9 gene editing. However, using a variety of assays, we did not observe significant differences in egg-laying behavior between <i>twk-26</i> and <i>twk-46</i> mutants and wild-type worms <i>.</i></p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811770/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001477","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Two-pore domain potassium channels, also known as K2P channels, play vital roles in maintaining the resting membrane potential in excitable cells, affecting a variety of physiological processes across species. The Caenorhabditis elegans ( C. elegans ) genome contains 46 different K2P-encoding genes, yet most of their functions remain unknown. Here, we have investigated the possible roles of two C. elegans K2P channel genes - twk-26 and twk-46 - that are expressed in the egg-laying neural circuit by characterizing the egg-laying behavior of null mutants generated by CRISPR/Cas9 gene editing. However, using a variety of assays, we did not observe significant differences in egg-laying behavior between twk-26 and twk-46 mutants and wild-type worms .
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