Quantifying chlorophylls in melanic lichens: the necessity of separating the absorbance of melanin and chlorophyll.

IF 2.9 3区 生物学 Q2 PLANT SCIENCES
Knut Asbjørn Solhaug, Yngvar Gauslaa
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引用次数: 0

Abstract

This study investigates extraction and quantification techniques for chlorophylls (Chl) in melanic lichens, with an emphasis on distinguishing between Chl and melanin absorbance during spectrophotometric assessments. We compared various extraction protocols, involving solvents such as dimethyl sulfoxide (DMSO) and acetone, and methods including intact thalli extraction, mortar grinding, and ball mill pulverizing. Three correction methods for melanic absorbance were also compared. Our findings indicated that DMSO was superior for Chl extraction compared to acetone, and differences in efficiency among the DMSO methods were minor. Correction for co-extracted melanin was deemed vital for accurate Chl quantification. The use of a C18 minicolumn was found to be effective for separating Chl and melanic pigments, providing reliable measurements of total Chl and Chl a/b-ratios. This method offers a simple cost-effective approach for Chl quantification in extracts containing a mix of Chl and other red light-absorbing pigments.

定量测定黑地衣中叶绿素含量:分离黑叶绿素吸光度的必要性。
本研究研究了黑地衣中叶绿素(Chl)的提取和定量技术,重点研究了在分光光度法评估中区分叶绿素和黑色素吸光度的方法。我们比较了各种提取方案,包括溶剂如二甲基亚砜(DMSO)和丙酮,以及包括完整菌体提取、砂浆研磨和球磨机粉碎在内的方法。比较了三种校正方法对黑素吸光度的影响。结果表明,DMSO萃取Chl的效果优于丙酮,且两种方法的萃取效率差异不大。对共提取黑色素的校正被认为对准确的Chl定量至关重要。使用C18微型色谱柱可有效分离Chl和黑素,提供可靠的总Chl和Chl a/b比值。该方法为含有Chl和其他红色吸光色素混合物的提取物中Chl的定量提供了一种简单、经济的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Photosynthesis Research
Photosynthesis Research 生物-植物科学
CiteScore
6.90
自引率
8.10%
发文量
91
审稿时长
4.5 months
期刊介绍: Photosynthesis Research is an international journal open to papers of merit dealing with both basic and applied aspects of photosynthesis. It covers all aspects of photosynthesis research, including, but not limited to, light absorption and emission, excitation energy transfer, primary photochemistry, model systems, membrane components, protein complexes, electron transport, photophosphorylation, carbon assimilation, regulatory phenomena, molecular biology, environmental and ecological aspects, photorespiration, and bacterial and algal photosynthesis.
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