Poppy Oldroyd, Santiago Velasco-Bosom, Sophia L Bidinger, Tawfique Hasan, Alexander J Boys, George G Malliaras
{"title":"Fabrication of thin-film electrodes and organic electrochemical transistors for neural implants.","authors":"Poppy Oldroyd, Santiago Velasco-Bosom, Sophia L Bidinger, Tawfique Hasan, Alexander J Boys, George G Malliaras","doi":"10.1038/s41596-024-01116-6","DOIUrl":null,"url":null,"abstract":"<p><p>Bioelectronic medicine, which involves the delivery of electrical stimulation via implantable electrodes, is poised to advance the treatment of neurological conditions. However, current hand-made devices are bulky, invasive and lack specificity. Thin-film neurotechnology devices can overcome these disadvantages. With a typical thickness in the range of micrometers, thin-film devices demonstrate high conformability, stretchability, are minimally invasive and can be fabricated using traditional lithography techniques. Despite their potential, variability and unreliability in fabrication processes hinder their wider utilization. Here, we detail a fabrication method for thin-film poly(ethylene dioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) electrodes and organic electrochemical transistors. The use of organic materials makes these devices particularly well suited for bioelectronic medicine applications as they show superior mechanical and electrical matching of biological tissues compared with devices made of inorganic materials. The procedure details the entire process, including mask design, the fabrication through three photolithography stages, the integration with larger-scale electronics, implantation procedures and the expected electrical characterization metrics. The nanofabrication protocol requires at least 3 d and is suitable for those familiar with lithographic fabrication procedures. The surgery requires up to 10 h and is suitable for those familiar with in vivo implantation procedures.</p>","PeriodicalId":18901,"journal":{"name":"Nature Protocols","volume":" ","pages":""},"PeriodicalIF":13.1000,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Protocols","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41596-024-01116-6","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Bioelectronic medicine, which involves the delivery of electrical stimulation via implantable electrodes, is poised to advance the treatment of neurological conditions. However, current hand-made devices are bulky, invasive and lack specificity. Thin-film neurotechnology devices can overcome these disadvantages. With a typical thickness in the range of micrometers, thin-film devices demonstrate high conformability, stretchability, are minimally invasive and can be fabricated using traditional lithography techniques. Despite their potential, variability and unreliability in fabrication processes hinder their wider utilization. Here, we detail a fabrication method for thin-film poly(ethylene dioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) electrodes and organic electrochemical transistors. The use of organic materials makes these devices particularly well suited for bioelectronic medicine applications as they show superior mechanical and electrical matching of biological tissues compared with devices made of inorganic materials. The procedure details the entire process, including mask design, the fabrication through three photolithography stages, the integration with larger-scale electronics, implantation procedures and the expected electrical characterization metrics. The nanofabrication protocol requires at least 3 d and is suitable for those familiar with lithographic fabrication procedures. The surgery requires up to 10 h and is suitable for those familiar with in vivo implantation procedures.
期刊介绍:
Nature Protocols focuses on publishing protocols used to address significant biological and biomedical science research questions, including methods grounded in physics and chemistry with practical applications to biological problems. The journal caters to a primary audience of research scientists and, as such, exclusively publishes protocols with research applications. Protocols primarily aimed at influencing patient management and treatment decisions are not featured.
The specific techniques covered encompass a wide range, including but not limited to: Biochemistry, Cell biology, Cell culture, Chemical modification, Computational biology, Developmental biology, Epigenomics, Genetic analysis, Genetic modification, Genomics, Imaging, Immunology, Isolation, purification, and separation, Lipidomics, Metabolomics, Microbiology, Model organisms, Nanotechnology, Neuroscience, Nucleic-acid-based molecular biology, Pharmacology, Plant biology, Protein analysis, Proteomics, Spectroscopy, Structural biology, Synthetic chemistry, Tissue culture, Toxicology, and Virology.