A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis.

Q2 Health Professions
Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari
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引用次数: 0

Abstract

Bacterial colonization of the cystic fibrosis (CF) airways is polymicrobial and several emerging microorganisms with a potential pathogenic role may be present. We report a case of three siblings with CF colonized by Pandoraea over a period of 10 years. Isolates identified as various non-fermentative Gram-negative bacilli from sputum cultures were successfully re-identified as Pandoraea vervacti (P. vervacti) by a combination of matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA and gyrB sequencing. Furthermore, the transcript expression of type I and type III interferon (IFN) genes was examined in the cells of respiratory samples from these patients and compared with a Pandoraea-negative group of CF individuals. Increased respiratory levels of IFNβ, IFNε and IL28R1 mRNA were found in the three siblings. Our results demonstrate that P. vervacti can chronically colonize CF patients and alter IFN response, likely contributing to immunopathogenesis and disease progression.

囊性纤维化患者持续性vervaci潘多拉菌感染的家族聚集性。
囊性纤维化(CF)气道的细菌定植是多微生物的,可能存在几种具有潜在致病作用的新兴微生物。我们报告一个病例的三个兄弟姐妹CF殖民化的Pandoraea超过10年。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)和16S rRNA和gyrB测序,成功地将痰培养物中分离的多种非发酵革兰氏阴性杆菌重新鉴定为vervacti Pandoraea (P. vervacti)。此外,在这些患者的呼吸样本细胞中检测了I型和III型干扰素(IFN)基因的转录表达,并与潘多拉阴性组CF个体进行了比较。在三个兄弟姐妹中发现IFNβ, IFNε和IL28R1 mRNA的呼吸水平升高。我们的研究结果表明,vervaci可以长期定殖CF患者并改变IFN反应,可能有助于免疫发病和疾病进展。
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来源期刊
CiteScore
3.50
自引率
0.00%
发文量
38
审稿时长
>12 weeks
期刊介绍: The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.
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