Glucose Oxidase Promoting Cellulase-Assisted Hydrodistillation for the Extraction of Essential Oil From Eleutherococcus Senticosus.

Abstract

Eleutherococcus senticosus essential oil (ESEO) has the function of clearing heat and detoxifying, delaying aging. The ESEO yield obtained by traditional extraction methods is low because essential oils are encased in plant cell structures and are difficult to extract. In this work, we adopted the method of glucose oxidase promoting cellulase-assisted hydrodistillation, and utilized the interaction of glucose oxidase and cellulase to promote the full destruction of cellulose in plant cells, resulting in a large amount of internal essential oil flowing out and improving the yield of ESEO. By optimizing the main factors of enzymatic hydrolysis, the optimal extraction conditions for ESEO were determined through Box-Behnken design experiments. The ratio of glucose oxidase to cellulase is 1:20, the concentration of the enzyme is 1.02%, the temperature of enzymatic hydrolysis is 40.0°C, and the pH value of enzymatic hydrolysis is 3.44. The optimal yield was 0.140%. The yield of essential oil extracted from E. senticosus was increased by 49.9% compared to a single cellulase-assisted extraction method. The extraction process used in this work is milder, more environmentally friendly, and more efficient, which has important reference value for the industrial extraction and utilization of medicinal plants.