Lateral-Flow Antibody Detection Device for the Diagnosis of Human Fascioliasis Using Simulated Whole-Blood Samples.

IF 1.9 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Patcharaporn Boonroumkaew, Lakkhana Sadaow, Nongnapas Kanchanangkul, Rutchanee Rodpai, Oranuch Sanpool, Hiroshi Yamasaki, Pewpan M Intapan, Wanchai Maleewong
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引用次数: 0

Abstract

Fascioliasis, which is caused by Fasciola hepatica and Fasciola gigantica (F. gigantica), is a public health problem worldwide. Stages infective to humans occur on aquatic plants and in water. The gold standard for diagnosing human infection depends on finding fluke eggs in stool samples. However, this method has low diagnostic sensitivity; eggs from other trematode species might be misidentified as those of Fasciola, and eggs are not voided in ectopic infections. Therefore, serological analysis can support the diagnosis of human fascioliasis. We evaluated whether a new fascioliasis immunochromatographic test kit, which detects specific IgG antibodies using F. gigantica excretory-secretory antigen, can be used for rapid diagnosis from whole blood. The kit ("the fascioliasis whole-blood test kit") was evaluated under laboratory conditions using 250 whole-blood samples (WBSs), of which 41 were from fascioliasis cases. Results based on simulated WBSs and the corresponding serum samples were compared and showed almost perfect agreement (percent agreement = 97.2%; kappa value = 0.9145). The diagnostic sensitivity and specificity of the test using simulated WBSs were 97.6% and 96.2%, respectively. Comparable values when serum samples were tested were 100.0% and 93.3%, respectively. This kit represents a significant advance because it does not require extensive training of personnel, is easy to use, and can support diagnosis at the bedside or in local and remote hospitals with limited facilities. The kit may also contribute significantly to epidemiological surveys.

使用模拟全血样本诊断人类法氏囊病的侧流抗体检测装置。
由肝片形吸虫和巨型片形吸虫引起的片形吸虫病是一个全球性的公共卫生问题。感染人类的阶段发生在水生植物和水中。诊断人类感染的黄金标准取决于在粪便样本中发现吸虫卵。但该方法诊断灵敏度较低;来自其他吸虫物种的卵可能被误认为是片形吸虫的卵,并且卵在异位感染中不会被排出。因此,血清学分析可支持人片吸虫病的诊断。我们评估了一种新的片形吸虫病免疫层析检测试剂盒是否可以用于全血快速诊断,该试剂盒使用巨形吸虫病排泄-分泌抗原检测特异性IgG抗体。该试剂盒(“片形吸虫病全血检测试剂盒”)在实验室条件下使用250份全血样本(WBSs)进行评估,其中41份来自片形吸虫病病例。基于模拟WBSs的结果与相应的血清样本进行比较,结果几乎完全一致(百分比一致性= 97.2%;Kappa值= 0.9145)。模拟wbs的诊断敏感性和特异性分别为97.6%和96.2%。血清样本检测的可比值分别为100.0%和93.3%。该试剂盒是一项重大进步,因为它不需要对人员进行大量培训,易于使用,并且可以在病床旁或在设施有限的地方和偏远医院进行诊断。该工具包还可对流行病学调查作出重大贡献。
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来源期刊
American Journal of Tropical Medicine and Hygiene
American Journal of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生
CiteScore
6.20
自引率
3.00%
发文量
508
审稿时长
3 months
期刊介绍: The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine. The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development. The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal. Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries
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