Mohamed A. Elsayed, Faisal K. Algethami, Abdullah N. Alotaibi, Huda Salem AlSalem, Lobna Mohammed Abd Elhalim, Mohammed Gamal
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引用次数: 0
Abstract
This paper represents an effective and reliable high-performance liquid chromatography-diode array detector (HPLC-DAD) method for the regular assay of Clorsulon (CLR) and Moxidectin (MOX) anti-parasitic drugs in injection solution and pure powder without derivatization processes. The mobile phase was composed of acetonitrile: methanol: water: acetic acid (56.0: 36.0: 7.5: 0.5 by volume). Besides, a Supelcosil C18 (4.60 mm ×15.0 cm, 5.0 μm) column was selected for completing the separation and quantitation of the two aforementioned veterinary drugs at a wavelength of 254 nm. The flow rate was set at 2.0 mL min−1 at the isocratic approach. We have conducted the degradation experiments using the HPLC-DAD instrument, adhering to the guidelines of the International Conference of Harmonization (ICH), subjecting CLR and MOX to light, heat, basic, acidic, and oxidative stressful conditions to figure out the ideal storage conditions and the possible medications that can be co-administered with them. CLR and MOX were quantified linearly from 400.0 to 1200.0 and from 40.0 to 120.0 µg mL−1, respectively. The maximum recorded degradation results were in acidic, basic, and oxidative conditions. Therefore, strong basic or acidic medications and oxidants shouldn’t be combined with CLR and MOX in a co-formulated medication. Greenness, carbon footprint, and blueness assessments for the novel method were conducted to verify the sustainability and functionality. The thirteen subdivisions in the GAPI pictogram, which were categorized as either green or yellow, refer to moderate green aspects. The final AGREE score of 0.56 and the majority of its subdivisions, ranging from dark green to yellow, indicated a relatively moderate level of greenness. This was primarily due to the significant acetonitrile content (56%) in the mobile phase. Using the HEXAGON method, the ultimate score is 0 out of 5 since the total calculated carbon footprint is less than 0.10. An eco-friendly method is one with a reduced carbon footprint score. The innovative HPLC method’s functioning and utility are indicated via its overall BAGI score of 80.0. Generally, the outcomes of the AGREE and GAPI pictograms indicate that the HPLC-DAD has a greenness feature, despite its moderate sensitivity.
本文建立了一种高效液相色谱-二极管阵列检测器（HPLC-DAD）方法，可用于抗寄生虫药物克罗舒隆（CLR）和莫西丁素（MOX）注射液和纯粉剂的常规检测，无需衍生化过程。流动相为乙腈：甲醇：水：乙酸（体积比为56.0:36.0:7.5:0.5）。选择superelcosil C18 （4.60 mm ×15.0 cm, 5.0 μm）色谱柱，在254 nm波长下完成上述两种兽药的分离和定量。流速设为2.0 mL min - 1。我们按照国际协调会议（ICH）的指导方针，使用HPLC-DAD仪器进行了降解实验，将CLR和MOX置于光、热、碱性、酸性和氧化应激条件下，以确定理想的储存条件和可能与它们共同使用的药物。CLR和MOX分别在400.0 ~ 1200.0和40.0 ~ 120.0µg mL−1范围内呈线性定量。记录的最大降解结果是在酸性、碱性和氧化条件下。因此，强碱性或酸性药物和氧化剂不应与CLR和MOX联合使用。对新方法进行了绿色、碳足迹和蓝色评估，以验证可持续性和功能性。GAPI象形图中的13个细分，被分类为绿色或黄色，指的是中度绿色方面。最终的AGREE得分为0.56，其大部分细分范围从深绿色到黄色，表明绿色水平相对中等。这主要是由于流动相中显著的乙腈含量（56%）。使用HEXAGON方法，最终得分为0（满分5分），因为计算的总碳足迹小于0.10。生态友好的方法是减少碳足迹得分的方法。创新的高效液相色谱法的功能和实用性通过其总BAGI得分为80.0来表明。一般来说，AGREE和GAPI象形图的结果表明HPLC-DAD具有绿色特征，尽管它的灵敏度中等。图形抽象
期刊介绍：
BMC Chemistry, formerly known as Chemistry Central Journal, is now part of the BMC series journals family.
Chemistry Central Journal has served the chemistry community as a trusted open access resource for more than 10 years – and we are delighted to announce the next step on its journey. In January 2019 the journal has been renamed BMC Chemistry and now strengthens the BMC series footprint in the physical sciences by publishing quality articles and by pushing the boundaries of open chemistry.
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