Optimisation of yield, flavonoids, and antioxidant activity via ultrasound-assisted extraction of bamboo leaves from Dinochloa sublaevigata S. Dransf. (Wadan) in Sabah, Malaysia

Muhammad Zahirul Amin Zulkifli , Mohammad Amil Zulhilmi Benjamin , Muhammad Daniel Eazzat Mohd Rosdan , Aniza Saini , Nor Azizun Rusdi , Mohd Azrie Awang
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Abstract

Dinochloa sublaevigata S. Dransf., an endemic bamboo species in Sabah, Malaysia, is valued for its ethnobotanical applications. However, research on extraction methods for its bioactive compounds and their bioactivities remains limited. This study optimised the D. sublaevigata leaf extract (DSLE) using response surface methodology (RSM) and ultrasound-assisted extraction (UAE). Extraction parameters, including ethanol concentration, temperature, ultrasonic amplitude, solid-to-solvent ratio, and extraction time, were initially screened using one-factor-at-a-time (OFAT) analysis to evaluate crude extract yield (CEY), total flavonoid content (TFC), and antioxidant capacity through 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Optimisation through a central composite design under RSM focused on temperature, amplitude, and solid-to-solvent ratio, while ethanol concentration (70 %) and extraction time (20 min) were fixed. The optimised conditions for DSLE—temperature (47.65 °C), amplitude (35.18 %), and solid-to-solvent ratio (1:25.11 g/mL)—achieved a CEY of 9.07 ± 0.13 %, TFC of 14.75 ± 0.03 mg RE/g extract, and DPPH of 91.78 ± 0.00 %. These results highlight a sustainable and efficient UAE method, establishing D. sublaevigata as a promising source for pharmaceutical and nutraceutical applications.
超声波辅助提取竹叶中黄酮类化合物及抗氧化活性的优化。(Wadan)在马来西亚的沙巴
亚种水蛭。是马来西亚沙巴州特有的竹种,具有民族植物学应用价值。然而,对其生物活性成分的提取方法及其生物活性的研究仍然有限。本研究采用响应面法(RSM)和超声辅助提取法(UAE)对紫叶提取物(DSLE)进行了优化。提取参数包括乙醇浓度、温度、超声振幅、固液比和提取时间,通过单因素一次(OFAT)分析来评估粗提物得率(CEY)、总黄酮含量(TFC)和2,2-二苯基-1-吡啶酰肼(DPPH)清除活性的抗氧化能力。在固定乙醇浓度(70 %)和提取时间(20 min)的条件下,通过RSM下的中心复合设计优化温度、振幅和固液比。DSLE-temperature的优化条件(47.65°C),振幅(35.18 %),和solid-to-solvent比(1:25.11 g / mL)研究者组成的CEY 9.07±0.13  %, 交通14.75±0.03  毫克/ g提取,和DPPH 91.78±0.00  %。这些结果强调了一种可持续和高效的UAE方法,确立了亚蓝叶紫薇作为一种有前途的制药和营养保健应用来源。
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CiteScore
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