High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC) (Adv. Mater. 6/2025)

Abstract

Extracellular Protein Identification Cytometry

Knowledge of the extracellular matrix drives our understanding of cell behavior. However, current analysis methods are limited to either bulk or low-throughput single-cell analysis, thus masking the heterogeneity in matrix deposition. Extracellular protein identification cytometry (EPIC) combines the high-throughput single-cell analysis of flow cytometry with engineered microniche 3D cell culture, jointly enabling in situ matrix analysis of large cell populations. More details can be found in article number 2415981 by Jeroen Leijten and co-workers.